Research Express | Hōʻike ka noiʻi ʻana o ke Kulanui ʻo Tsinghua hiki i ka NMN ke mālama i ka mumū

ʻO ka hoʻoulu ʻana o ka macrophage kahi ʻano pathogenic e alakaʻi ana i ka mumū mau loa i loko o ke kino, akā ʻo ka hoʻoulu mau ʻana o ka macrophage hiki ke alakaʻi i ka mumū mau loa a me nā maʻi e like me ke kūʻē ʻana o ka insulin a me nā maʻi koʻikoʻi e like me ka atherosclerosis. ʻO PGE 2, ka mea e hoʻoponopono i ka pane mumū, ua synthesized ʻia mai ka waikawa arachidonic e nā cyclooxygenases (COX-1 a me COX-2). ʻO COX-1 a me COX-2 nā pahuhopu nui o ka anti-inflammatory a hiki ke kāohi ʻia e nā lāʻau anti-inflammatory non-steroidal (NSAIDs).

Hiki i ka hoʻohana ʻana i nā NSAID ke hana i nā hopena maikaʻi ʻole he nui, e like me ke kahe koko ʻana o ka ʻōpū. No laila, he mea nui loa ka loaʻa ʻana o kahi mea kūlohelohe palekana e mālama ai i ka mumū.

I kēia mau lā iho nei, ua mālama kahi hui noiʻi mai ke Kulanui ʻo Tsinghua i nā macrophages ʻiole me NMN, a ua hōʻoia ma o nā hoʻokolohua e hiki i ka NMN ke hōʻemi i ka hōʻiliʻili ʻana o nā protein e pili ana i ka mumū a me nā huahana metabolic, a kāohi i ka pane mumū o nā macrophages. Nā Palena ma nā Biosciences Molecular.

Hoʻololi ka mumū i nā pae o nā huahana metabolic i nā macrophages
ʻO ka mea mua, ua hoʻāla ka hui noiʻi i nā macrophages e hana i ka mumū ma o lipopolysaccharide (LPS), a laila ua kālailai i ka ʻike o nā huahana a puni nā macrophages i ka wā o ka mumū. Ua hoʻonui ʻia nā pae o 99 metabolites a ua hoʻemi ʻia he 105 metabolites i waena o nā molekala 458 i ʻike ʻia ma mua a ma hope o ka hoʻoulu ʻana o ka mumū, a ua hoʻemi ʻia hoʻi nā pae NAD+ e hele pū me ka mumū.

(KIʻI 1)

Hoʻonui ka NMN i nā pae NAD a hoʻemi i ka mumū macrophage
A laila mālama ka hui noiʻi i nā macrophages me LPS, kahi e hoʻoulu ai i kahi kūlana mumū, IL-6 a me IL-1β, nā cytokines pro-inflammatory e hana ma ke ʻano he mau māka o ka mumū. Ma hope o ka mālama ʻana o NMN i ka mumū macrophage i hoʻokomo ʻia e LPS, ua ʻike ʻia ua hoʻonui ʻia ka pae NAD intracellular a ua emi ka hōʻike mRNA o IL-6 a me IL-1β. Ua hōʻike nā hoʻokolohua ua hoʻonui ʻo NMN i ka pae o NAD a hoʻēmi i ka mumū macrophage i hoʻokomo ʻia e LPS.

(Kiʻi 2)

(Kiʻi 3)

Hoʻemi ka NMN i nā pae protein e pili ana i ka mumū
I ka mālama ʻana i ka NMN, ua ʻike ʻia ua emi nā protein e pili ana i ka mumū e like me RELL1, PTGS2, FGA, FGB a me igkv12-44 i loko o nā pūnaewele, kahi i hōʻike ai ua hōʻemi ka NMN i ka hōʻike ʻana o nā protein e pili ana i ka mumū.

(Kiʻi 4)

Hoʻemi ka NMN i ka hōʻike ʻana o nā protein target NSAIDS
Ua ʻike ʻia ma ka hoʻokolohua hope loa ua hoʻemi ʻo NMN i ka pae o PGE2 i loko o nā pūnaewele RAW264.7 i hoʻāla ʻia e LPS ma ka hoʻemi ʻana i ka pae hōʻike o COX-2, a laila e hōʻemi ana i ka hōʻike ʻana o COX2 a me ka pale ʻana i ka mumū i hoʻokumu ʻia e LPS.

(Kiʻi 6)

hopena, hiki i ka hoʻohui ʻana o NMN ke mālama pono i ka mumū mau loa i nā ʻiole, a pono e hōʻoia ʻia ka mālama ʻana i ka mumū i loko o ke kanaka e nā hoʻokolohua lapaʻau pili. Malia paha e lilo ʻo NMN i pani no NSAIDS i ka wā e hiki mai ana.

nā kuhikuhi:
1.Liu J, Zong Z, Zhang W, Chen Y, Wang X, Shen J, Yang C, Liu X, Deng H. ʻO Nicotinamide Mononucleotide Hoʻopau i ka LPS-Induced Inflammation and Oxidative Stress ma o ka ho'ēmiʻana i ka COX-2 Expression i Macrophages. Mua Mol Biosci. 2021 Iulai 6.