CALB i hoʻopaʻa ʻia
Hoʻopaʻa ʻia ʻo CALB e ka adsorption kino ma luna o ka resin hydrophobic kiʻekiʻe he polymer macroporous, styrene/methacrylate. He kūpono ka CALB i hoʻopaʻa ʻia no nā noi i nā mea hoʻoheheʻe organik a me nā ʻōnaehana solvent-free, a hiki ke hana hou ʻia a hoʻohana hou ʻia no nā manawa he nui ma nā kūlana kūpono.
Huahana Huahana: SZ-CALB- IMMO100A, SZ-CALB- IMMO100B.
★ʻOi aku ka hana kiʻekiʻe, ʻoi aku ka koho chiral kiʻekiʻe a me ke kūpaʻa kiʻekiʻe.
★Hana maikaʻi aʻe i nā pae ʻaʻohe wai.
★E wehe maʻalahi mai ka ʻōnaehana hana, e hoʻopau koke i nā hana, a pale i ke koena protein i loko o ka huahana.
★Hiki ke hana hou ʻia a hoʻohana hou ʻia e hōʻemi i ke kumukūʻai.
| Hana | ≥10000PLU/g |
| pae pH no ka hopena | 5-9 |
| Pae mahana no ka hopena | 10-60℃ |
| helehelena | CALB-IMMO100-A: Melemele māmā a ʻeleʻele paʻa CALB-IMMO100-B: Keʻokeʻo a ʻeleʻele māmā paʻa |
| Ka nui o ka ʻāpana | 300-500μm |
| Pohō ma ka maloʻo ʻana ma 105 ℃ | 0.5%-3.0% |
| ʻO ka Resin no ka hoʻopaʻa ʻana | Polymer styrene/methacrylate macroporous |
| Mea hoʻoheheʻe hopena | Wai, mea hoʻoheheʻe organik, a pēlā aku, a i ʻole me ka ʻole o ka mea hoʻoheheʻe. No ka hana ʻana i loko o kekahi mau mea hoʻoheheʻe organik, hiki ke hoʻohui ʻia he 3% wai e hoʻomaikaʻi i ka hopena o ka hana. |
| Ka nui o ka ʻāpana | CALB-IMMO100-A: 200-800 μm CALB-IMMO100-B: 400-1200 μm |
Wehewehe ʻana o ka ʻāpana: Hoʻohālikelike ka ʻāpana 1 i ka hana ʻana o 1μmol i kēlā me kēia minuke propyl laurate mai ka waikawa lauric a me 1-propanol ma 60 ℃. ʻO CALB-IMMP100-A a me CALB-IMMO100-B i luna e pili ana i nā mea lawe paʻa me nā nui ʻāpana like ʻole.
1. ʻAno reactor
Hoʻohana ʻia ka enzyme paʻa i ka reactor batch kettle a me ka reactor kahe mau o ka moena paʻa. Pono e hoʻomaopopo ʻia e pale i ka wāwahi ʻia ma muli o ka ikaika o waho i ka wā e hānai ana a hoʻopiha paha.
2. pH pane, mahana a me ka mea hoʻoheheʻe
Pono e hoʻohui hope ʻia ka enzyme i hoʻopaʻa ʻia, ma hope o ka hoʻohui ʻia ʻana o nā mea ʻē aʻe a me ka hoʻoheheʻe ʻia, a hoʻoponopono ʻia ka pH.
Inā ʻo ka hoʻopau ʻana o ka substrate a i ʻole ka hoʻokumu ʻia ʻana o ka huahana e alakaʻi i ka loli o ka pH i ka wā o ka hopena, pono e hoʻohui ʻia ka buffer lawa i ka ʻōnaehana hopena, a i ʻole e nānā ʻia a hoʻoponopono ʻia ka pH i ka wā o ka hopena.
I loko o ka pae hoʻomanawanui mahana o CALB (ma lalo o 60 ℃), ua piʻi ka helu hoʻololi me ka piʻi ʻana o ka mahana. I ka hoʻohana pono ʻana, pono e koho ʻia ka mahana hopena e like me ke kūpaʻa o ka substrate a i ʻole ka huahana.
Ma keʻano laulā, kūpono ka hopena hydrolysis ester i loko o ka ʻōnaehana pae wai, ʻoiai kūpono ka hopena synthesis ester i loko o ka ʻōnaehana pae organik. Hiki i ka mea hoʻoheheʻe organik ke ethanol, tetrahydrofuran, n-hexane, n-heptane a me toluene, a i ʻole kahi mea hoʻoheheʻe hui kūpono. No ka hopena i loko o kekahi mau mea hoʻoheheʻe organik, hiki ke hoʻohui ʻia he 3% wai e hoʻomaikaʻi i ka hopena o ka hopena.
3. Hoʻohana hou a me ke ola lawelawe o CALB
Ma lalo o ke kūlana kūpono o ka hopena, hiki ke hoʻihoʻi ʻia a hoʻohana hou ʻia ʻo CALB, a ʻokoʻa nā manawa noi kikoʻī me nā papahana like ʻole.
Inā ʻaʻole hoʻohana hou ʻia ka CALB i hoʻōla ʻia a pono e mālama ʻia ma hope o ka hoʻōla ʻana, pono e holoi ʻia a hoʻomaloʻo ʻia a hoʻopaʻa ʻia ma 2-8 ℃.
Ma hope o kekahi mau hoʻohana hou ʻana, inā ua emi iki ka pono o ka hopena, hiki ke hoʻohui ʻia ʻo CALB i kahi kūpono a hoʻomau i ka hoʻohana ʻana. Inā ua emi nui ka pono o ka hopena, pono e hoʻololi ʻia.
Laʻana 1 (Aminolysis)(1):
Laʻana 2 (Aminolysis)(2):
Laʻana 3 (Hana ʻia ka polyester wehe apo)(3):
Laʻana 4 (Transesterification, regioselective o ka hui hydroxyl)(4):
Laʻana 5 (Transesterification, ka hoʻonā kinetic o nā waiʻona racemic)(5):
Laʻana 6 (Esterification, hoʻonā kinetic o ka waikawa carboxylic)(6):
Laʻana 7 (Esterolysis, hoʻonā kinetic)(7):
Laʻana 8 (Hydrolysis o nā amides)(8):
Laʻana 9 (Acylation o nā amines)(9):
Laʻana 10 (ka hopena hoʻohui ʻo Aza-Michael)(10):
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