Phosphokinase (PKase)
ʻO ka wehewehe pōkole:
E pili ana i ka Phosphokinase
ES-PKase (Phosphokinase): hoʻopale i ka hoʻololi ʻana o nā pūʻulu phosphate ma ATP i nā pūhui ʻē aʻe.Hoʻopili pū ia i ka hoʻoili ʻana o nā pūʻulu phosphate ma nā nucleosides o triphosphate i kekahi manawa.Pono ka hapa nui o nā kinase i nā ion metala divalent no ke komo ʻana i ka hopena (maʻamau Mg2+).He 21 ʻano huahana enzyme PKase (Helu e like me ES-PKase101~ ES-PKase-121) i hoʻomohala ʻia e SyncoZymes.
ʻAno hoʻopā catalytic:
Mobile/Wechat/WhatsApp: +86-13681683526
E-mail:lchen@syncozymes.com
| Nā Enzymes | Code Huahana | Hōʻike |
| Mea Hoʻomaʻamaʻa (SynKit) | ES-PKase-101~ES-PKase-121 | he set o 21 Ketoreductases, 1 mg i kēlā me kēia 21 mau mea * 1mg / mea |
★ High substrate specificity.
★ Ke koho kiral ikaika.
★ hoʻololi kiʻekiʻe.
★ Liʻiliʻi nā huahana.
★ Nā kūlana hoʻonā haʻahaʻa.
★ Pili kaiapuni.
➢ ʻO ka mea maʻamau, pono e hoʻokomo ʻia ka ʻōnaehana hoʻololi i kahi substrate, solution buffer, enzyme, ATP, Mg2+.
➢ Pono e hoʻohui hope ʻia ka PKase i loko o ka ʻōnaehana pane, ma hope o ka hoʻololi ʻana o ka pH a me ka mahana i ke ʻano o ka hopena.
Laʻana 1(Ka hoʻohui ʻana o ka nicotinamide ribose phosphate mai ka nicotinamide ribose)(1):
'Ōlelo Aʻo: No ka maʻalahi o ka hoʻomaopopo ʻana, ua manaʻo ʻia nā hiʻohiʻona noiʻi a me nā kuhikuhi e hōʻike i ke ʻano o ka noi ʻana o PKase no ka maʻalahi o ka hoʻomaopopo ʻana, a ʻaʻole kūpono i ka enzyme kikoʻī o SyncoZymes.
E mālama i 2 mau makahiki ma lalo o -20 ℃.
Mai hoʻopili i nā kūlana koʻikoʻi e like me: ka wela kiʻekiʻe, ka pH kiʻekiʻe/haʻahaʻa a me ka mea hoʻoheheʻe kūlohelohe kiʻekiʻe.
1. Khan, Javed A., Song Xiang, a me Liang Tong.Kūlana 15.8 (2007): 1005-1013.
