ʻO ka phenylalanine dehydrogenase (PDH)
Wehewehe Pōkole:
E pili ana i ka Phenylalanine dehydrogenase
ES-PDH: He papa o ka oxidoreductase. Hiki i ka hopena maikaʻi ke hoʻoulu i ka deamination oxidative o ka paʻakai L phenylalanine i ke alo o NAD e hana i ka paʻakai phenylpyruvate, a hiki i ka hopena hope ke hoʻoulu i ka synthesis o ka paʻakai amino acid like mai ka paʻakai phenylpyruvate. Ua hoʻomohala ʻo Syncozymes i 8 mau mea o ka phenylalanine dehydrogenase (i helu ʻia ʻo ES-PDH-101~ES-PDH-108). Hiki ke hoʻohana ʻia ʻo ES-PDH e hoʻomākaukau i ka paʻakai phenylalanine a i ʻole ka paʻakai amino acid like mai ka paʻakai phenylpyruvic acid a i ʻole nā mea i loaʻa.
ʻAno hana catalytic:
Kelepona Paʻalima/Wechat/WhatsApp: +86-13681683526
Leka uila:lchen@syncozymes.com
| Nā ʻenzyme | Kōmike Huahana | Nā kikoʻī |
| Pauka ʻenzyme | ES-PDH-101~ ES-PDH-108 | he set o 8 Nitrile Reductase, 50 mg kēlā me kēia 8 mau mea * 50mg / mea, a i ʻole ka nui ʻē aʻe |
★ Kiʻekiʻe ke ʻano kikoʻī o ka substrate.
★ Koho chiral ikaika.
★ Hoʻololi kiʻekiʻe.
★ Emi nā huahana ʻaoʻao.
★ Nā kūlana pane iki.
★ Mālama i ke kaiapuni.
➢ ʻO ka maʻamau, pono i ka ʻōnaehana hana ke komo pū me ka substrate, ka hopena buffer, ka enzyme, ka coenzyme (NAD(H)), ka ʻōnaehana hana hou ʻana o ka coenzyme (e laʻa me ka ammonium formate a me ka formate dehydrogenase).
➢ ʻO kēlā me kēia ʻano ES-PDH e pili ana i nā kūlana hopena kūpono like ʻole, hiki ke aʻo ʻia pākahi inā pono.
➢ Pono e hoʻohui hope ʻia ka ES-PDH i loko o ka ʻōnaehana hana e mālama i ka hana.
Laʻana 1 (Biosynthesis o nā phenylalanine derivatives mai nā phenylpyruvate derivatives)(1):
E mālama no 2 mau makahiki ma lalo o -20℃.
Mai hoʻopili aku me nā kūlana koʻikoʻi e like me: ke kiʻekiʻe o ka mahana, ka pH kiʻekiʻe/haʻahaʻa a me ka mea hoʻoheheʻe organik kiʻekiʻe.
1. Francesca Paradisi, Stuart Collins, et al. Nūpepa o ka Biotechnology, 2007, 128, 408–411.
