Ene reductase (ERED)
Hoʻopili nā ES-ERED i nā ʻano substrate like ʻole ma muli o ka laulā ākea o nā substrate.Ma keʻano laulā,ʻo ka C = C o α, β -unsaturated pūhui me nā pūʻulu electron-absorbing (me ketone aldehyde, nā hui nitro, carboxylic acids, esters, anhydride, lactones, imines, a me nā mea'ē aʻe) hiki ke hoʻemi maʻalahi e ES-EREDs, akā. ʻaʻole nā mea paʻa paʻa ʻole.
Aia he 46 ʻano huahana enzyme ERED (Helu e like me ES-ERED-101~ES-ERED-146) i hoʻomohala ʻia e SyncoZymes.
ʻO ka mīkini hoʻoheheʻe:
| Nā Enzymes | Code Huahana | Hōʻike |
| ʻO ka pauka Enzyme | ES-ERED-101~ ES-ERED-146 | he set o 46 Ene Reductases, 50 mg kēlā me kēia 46 mau mea * 50mg / mea, a i ʻole ka nui ʻē aʻe |
| Mea Hoʻomaʻamaʻa (SynKit) | ES-ERED-4600 | he set o 46 Ene Reductases, 50 mg kēlā me kēia 46 mau mea * 50mg / mea, a i ʻole ka nui ʻē aʻe |
★ High substrate specificity.
★ Ke koho kiral ikaika.
★ hoʻololi kiʻekiʻe.
★ Liʻiliʻi nā huahana.
★ Nā kūlana hoʻonā haʻahaʻa.
★ Pili kaiapuni.
★ Palekana kiʻekiʻe.
➢ ʻO ka mea maʻamau, pono e hoʻokomo ʻia ka ʻōnaehana hoʻololi i ka substrate, ka solution buffer (pH hopena maikaʻi loa), nā coenzymes (NAD(H) a i ʻole NADP(H) ), ʻōnaehana hoʻoulu hou coenzyme (eg glucose a me ka glucose dehydrogenase) a me ES-ERED.
➢ Hiki ke ho'āʻo ʻia nā ES-ERED a pau ma ka ʻōnaehana pane ma luna a i ʻole me ka ERED Screening Kit (SynKit ERED).
➢ Pono e aʻo pākahi ʻia nā ʻano ES-ERED a pau e pili ana i nā ʻano ʻano hopena maikaʻi loa.
➢ Hiki ke kaohi i ka hana a ES-ERED.Eia naʻe, hiki ke hoʻokuʻu ʻia ka inhibition me ka hoʻohui pū ʻana o ka substrate.
Laʻana 1(α,β-unsaturated aldehydes a i ʻole ketones)(1):
Ka Laʻana 2(α,β-unsaturated carboxylic acids a me kā lākou mau mea i loaʻa)(2):
E mālama i 2 mau makahiki ma lalo o -20 ℃.
Mai hoʻopili i nā kūlana koʻikoʻi e like me: ka wela kiʻekiʻe, ka pH kiʻekiʻe/haʻahaʻa a me ka mea hoʻoheheʻe kūlohelohe kiʻekiʻe.
1. Lucídio C, Fardelone J, Augusto R, e tal.J.Mol.Catal.B: Enzym., 2004, 29: 41-45.
2. Stueckler C, Hall M, Ehammer H, e tal..Org.Lett, 2007, 9(26): 5409-5411.
