Ketoreductase (KRED)
KRED mai SyncoZymes: Aia he 191 ʻano huahana enzyme KRED (Helu e like me ES-KRED-101~ES-KRED-291) i hoʻomohala ʻia e SyncoZymes.Hiki ke hoʻohana nui ʻia ka SZ-KRED i ka hoʻemi regio- a me stereoselective o nā aldehydes, beta-ketone esters, α-ketone esters a me nā ketones.
ʻAno hoʻopā catalytic:
| Nā Enzymes | Code Huahana | Hōʻike |
| ʻO ka pauka Enzyme | ʻO ka pauka Enzyme | he set o 187 Ketoreductases, 50 mg kēlā me kēia 187 mau mea * 50mg / mea, a i ʻole ka nui ʻē aʻe |
| Mea Hoʻomaʻamaʻa (SynKit) | ES-KRED-18100 | he set o 181 Ketoreductases, 1 mg i kēlā me kēia 181 mau mea * 1mg / mea |
★ High substrate specificity.
★ Ke koho kiral ikaika.
★ kiʻekiʻe hoʻololi kūpono.
★ Liʻiliʻi nā huahana.
★ Nā kūlana hoʻonā haʻahaʻa.
★ Pili kaiapuni.
➢ Pono e hoʻokō ʻia ka nānā ʻana i ka enzyme no nā substrate kikoʻī ma muli o ke ʻano kikoʻī o ka substrate, a e kiʻi i kahi enzyme e hoʻolalelale i ka substrate i manaʻo ʻia me ka hopena catalytic maikaʻi loa.
➢ Mai hoʻopili aku me nā kūlana koʻikoʻi e like me: ka wela kiʻekiʻe, ka pH kiʻekiʻe/haʻahaʻa a me ka mea hoʻoheheʻe organik me ka ʻike kiʻekiʻe.
➢ ʻO ka mea maʻamau, pono e hoʻokomo i ka ʻōnaehana hoʻololi i kahi substrate, solution buffer, coenzymes(NAD(H) a i ʻole NADP(H) ), coenzyme regeneration system (eg glucose and glucose dehydrogenase).
➢ Pono e hoʻohui hope ʻia ʻo KRED i loko o ka ʻōnaehana pane, ma hope o ka hoʻololi ʻana o ka pH a me ka mahana i ke ʻano o ka hopena.
➢ Loaʻa i nā ʻano KRED like ʻole ke ʻano o ka hopena maikaʻi loa, no laila pono e aʻo hou ʻia kēlā me kēia.
Laʻana 1(1):
Laʻana 2(2):
1. Li Z, Liu WD, Chen X, e tal.Tetrahedron, 2013, 69, 3561-3564.
2. Ni Y, Li CX, Ma HM, e tal.Appl Microbiol Biotechnol, 2011, 89: 1111-1118.
