Cytochrome P450 monooxygenase (CYP)

ʻO ka wehewehe pōkole:

E pili ana iā CYP

ES-CYPs: He papa o nā enzymes redox me mercaptan-heme ma ke ʻano he kikowaena hana e hoʻoikaika i ka hoʻoili oxygen.He mau oxygenases hope lākou a pono lākou i ka heme e hoʻāla ai i ka oxygen.

Cytochrome P450 monooxygenase catalyze i ka oxidation, epoxidation a me ka hydroxylation o alkyl, hydroxylation a me ka oxidation o ka amonia, oxidation o sulfur, dealkylation (oxygen, sulfur, amonia), oxidative dehydrogenation, deamination a me ka dehalogenation.
He 8 mau ʻano huahana cytochrome P450 monooxygenase (Helu e like me ES-CYP-101～ES-CYP-108) i hoʻomohala ʻia e SyncoZymes.

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E-mail:lchen@syncozymes.com

E hoʻouna i ka leka uila iā mākou

Huahana Huahana

E pili ana iā CYP:

ʻAno hoʻopā catalytic:

ʻIkepili Huahana:

Nā Enzymes	Mea Hoʻomaʻamaʻa (SynKit)	Hōʻike
ʻO ka pauka Enzyme	ES-CYP-101~ ES-CYP-108	he pūʻulu o 8 Cytochrome P450 Monooxygenases, 50 mg i kēlā me kēia 8 mau mea * 50mg / mea, a i ʻole ka nui ʻē aʻe
Mea Hoʻomaʻamaʻa (SynKit)	ES-CYP-800	kahi hoʻonohonoho o 8 Cytochrome P450 Monooxygenases, 1mg kēlā me kēia 8 mau mea * 1mg / mea

Pono:

★ ʻākeʻe substrate spectrum.
★ hoʻololi kiʻekiʻe.
★ Liʻiliʻi nā huahana.
★ Nā kūlana hoʻonā haʻahaʻa.
★ Pili kaiapuni.

Nā kuhikuhi no ka hoʻohana ʻana:

➢ ʻO ka mea maʻamau, pono e hoʻokomo i ka ʻōnaehana hoʻololi i ka substrate, ka solution buffer (The optimum reaction pH of enzyme), coenzyme (NAD(H) or NADP(H)), coenzyme regeneration system (eg glucose and glucose dehydrogenase) a me ka ES-CYP.Hiki ke pani ʻia ka ʻōnaehana coenzyme a me ka coenzyme e ka hydrogen peroxide i loko o ka ʻōnaehana pane ʻāpana.
➢ Pono e aʻo pākahi ʻia nā ʻano ES-CYP a pau e pili ana i nā ʻano hopena maikaʻi loa.
➢ ʻO ka substrate noʻonoʻo kiʻekiʻe a i ʻole nā huahana me ka hiki ke kaohi i ka hana a ES-CYP.Eia naʻe, hiki ke hoʻokuʻu ʻia ka inhibition me ka hoʻohui pū ʻana o ka substrate.

Nā Laʻana Noi:

Laʻana 1⁽¹⁾:

Laʻana 2⁽²⁾:

Laʻana 3⁽³⁾:

Laʻana 4⁽⁴⁾:

Waihona:

E mālama i 2 mau makahiki ma lalo o -20 ℃.

Nānā:

Mai hoʻopili i nā kūlana koʻikoʻi e like me: ka wela kiʻekiʻe, ka pH kiʻekiʻe/haʻahaʻa a me ka mea hoʻoheheʻe kūlohelohe kiʻekiʻe.

Kuhikuhi:

1. Zaretzki J, Matlock M, a me Swamidass S JJ Chem.Inf.Hoʻohālike, 2013, 53, 3373–3383.
2. Gannett P M., Kabulski J, Perez F A., e tal.J. Am.Kemi.Soc., 2006, 128 (26), 8374–8375.
3. Cryle M J., Matovic N J., a me De Voss J J. Org.Lett., 2003, 5 (18), 3341–3344.
4. Kawauchi, H., Sasaki, J., Adachi, T., e tal.Biochim.Biophys.Acta, 1994, 1219, 179.
5. Yasutake, Y., Fujii, Y.;Cheon, WK e tal.Acta Crystallogr.2009, 65, 372.