Transaminase (ATA)
Nā Enzymes: He macromolecular biological catalysts, ʻo ka hapa nui o nā enzyme he protein.
Nā Transaminases: He papa o nā enzymes e hoʻopololei ana i ka hoʻololi ʻana ma waena o nā waikawa amino a me nā waikawa keto.ʻO nā transaminases nā enzyme biological koʻikoʻi i ka synthesis asymmetric a me ka hoʻonā racemic o nā amine chiral.
Hiki ke hoʻokaʻawale ʻia ʻo Aminotransferase i ʻehā mau papa e like me ke kaʻina a me ke ʻano: Ⅰ, Ⅱ, Ⅲ a me Ⅳ.Aia nā ω-aminotransferases i ka papa II transaminases, hoʻohana mau ʻia i ka hoʻomākaukau ʻana i nā amine chiral a me nā waikawa amino kūlohelohe, e like me nā β-amino acids.
ω-aminotransferases: I ka hapanui o nā hihia, pili ka ω-transaminase i kahi papa o nā enzymes, ʻo ia ka hoʻololi ʻana o ka ammonia catalytic me ka ʻole α-amino acid ma ke ʻano he substrate a huahana paha.
ʻO ka mīkini hoʻoheheʻe:
| Nā Enzymes | Code Huahana | Code Huahana |
| ʻO ka pauka Enzyme | ES-ATA-101~ ES-ATA-165 | he set o 65 ω-Transaminases, 50 mg kēlā me kēia 65 mau mea * 50mg / mea, a i ʻole ka nui ʻē aʻe |
| Mea Hoʻomaʻamaʻa (SynKit) | ES-ATA-6500 | he set o 65 ω-Transaminases, 1 mg i kēlā me kēia 65 mau mea * 1mg / mea |
★ High substrate specificity.
★ Ke koho kiral ikaika.
★ kiʻekiʻe hoʻololi kūpono.
★ Liʻiliʻi nā huahana.
★ Nā kūlana hoʻonā haʻahaʻa.
★ Pili kaiapuni.
➢ Pono e hoʻokō ʻia ka nānā ʻana i ka enzyme no nā substrate kikoʻī ma muli o ke ʻano kikoʻī o ka substrate, a e kiʻi i kahi enzyme e hoʻolalelale i ka substrate i manaʻo ʻia me ka hopena catalytic maikaʻi loa.
➢ Mai hoʻopili aku me nā kūlana koʻikoʻi e like me: ka wela kiʻekiʻe, ka pH kiʻekiʻe/haʻahaʻa a me ka mea hoʻoheheʻe organik me ka ʻike kiʻekiʻe.
➢ ʻO ka mea maʻamau, pono e hoʻokomo i ka ʻōnaehana hoʻololi i ke substrate, ka solution buffer, ka mea hāʻawi amino (e like me nā amino acids a me 1-phenyl ethylamine) a i ʻole ka mea hoʻokipa (e like me keto acids), coenzyme (PLP), cosolvent (e like me DMSO).
➢ Pono e hoʻohui hope ʻia ʻo ATA i loko o ka ʻōnaehana pane, ma hope o ka hoʻololi ʻana o ka pH a me ka mahana i ke ʻano o ka hopena.
➢ Loaʻa i nā ʻano ATA a pau nā ʻano hopena maikaʻi loa, no laila pono e aʻo hou ʻia kēlā me kēia.
Laʻana 1 (ka hoʻohui ʻana o Sitagliptin, synthesis asymmetric)(1):
Laʻana 2 (Mexiletine, Hui ʻia o ka hoʻonā kinetic me ka synthesis asymmetric)(2):
1 Savile CK, Janey JM, Mundorff EC, et al.ʻEpekema, 2010, 329(16), 305-309.
2 Koszelewski D, Pressnitz D, Clay D, et al.Nā leka kino, 2009,11(21):4810-4812.
