Leucine Dehydrogenase (LeuDH)
ʻO ka wehewehe pōkole:
E pili ana i ka Leucine dehydrogenase
Aia 1 ʻano huahana enzyme LeuDH (Helu e like me ES-LeuDH-101) i hoʻomohala ʻia e SyncoZymes.Hiki i ka LeuDH ke hoʻololi i ka ammoniation reductive o ka 4-methylbutanoic acid (paʻakai) i ka L-leucine a i ʻole ka oxidation o ka leucine i ka 4-methylbutanoic acid.Hiki i ka ES-LeuDH ke hoʻoikaika i ka hoʻohaʻahaʻa ʻana o nā ʻakika α-keto me ke ʻano like e hoʻomākaukau ai i nā derivatives amino acid.I ke kaʻina hana o ka catalysis, pono ʻo NADH ma ke ʻano he hydrogen transporter.
Leucine dehydrogenasecatalytic reaction type
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E-mail:lchen@syncozymes.com
| Nā Enzymes | Code Huahana | Hōʻike |
| ʻO ka pauka Enzyme | ES-LeuDH-101 | he set o 1 Monoamine Oxidase, 50 mg i kēlā me kēia 1 mau mea * 50mg / mea, a i ʻole ka nui ʻē aʻe |
Hiki ke hoʻohana ʻia kēia ʻōnaehana pane no ke kuhikuhi ʻana:
1-10 mg/ml: Pāpā
10 mg/ml: Enzyme
10% (v/v): Mea hoʻoheheʻe kino (1)
100 mM: Hoʻopaʻa Phosphate (pH7.0)
0.2 mg/ml: NAD+
100 mM: Ammonium formate
10 mg/ml: Formate dehydrogenase (FDH)
Hoʻomoʻa i nā hopena ma kahi haʻalulu wela mau (no ka laʻana, 30oC, 150rpm) i loko no 24-48 mau hola.ʻO Centrifuge i kēlā me kēia hui e hoʻoheheʻe i ka protein, hiki ke hoʻohana ʻia ka supernatant no ka TLC, HPLC a i ʻole GC analysis.
Nānā (1): ʻO ka solvent organik (e like me DMSO, methanol, acetonitrile, IPA, etc.) he koho, hoʻohana ʻia no nā substrates insoluble.
