ʻO ka Leucine Dehydrogenase (LeuDH)
Wehewehe Pōkole:
E pili ana i ka Leucine dehydrogenase
Aia kekahi ʻano huahana enzyme LeuDH (Helu e like me ES-LeuDH-101) i hoʻomohala ʻia e SyncoZymes. Hiki iā LeuDH ke hoʻoulu i ka ammoniation reductive o ka 4-methylbutanoic acid (paʻakai) i L-leucine a i ʻole ka oxidation o ka leucine i 4-methylbutanoic acid. Hiki iā ES-LeuDH ke hoʻoulu i ka amination reductive o nā waikawa α-keto me ke ʻano like e hoʻomākaukau i nā derivatives amino acid e pili ana. I ke kaʻina hana o ka catalysis, pono ʻo NADH ma ke ʻano he mea lawe hydrogen.
ʻAno hopena catalytic Leucine dehydrogenase
Kelepona Paʻalima/Wechat/WhatsApp: +86-13681683526
Leka uila:lchen@syncozymes.com
| Nā ʻenzyme | Kōmike Huahana | Nā kikoʻī |
| Pauka ʻenzyme | ES-LeuDH-101 | he set o 1 Monoamine Oxidase, 50 mg kēlā me kēia 1 mau mea * 50mg / mea, a i ʻole ka nui ʻē aʻe |
Hiki ke hoʻohana ʻia ka ʻōnaehana hana ma lalo nei no ka ʻike:
1-10 mg/ml: Pākuʻi
10 mg/ml: ʻEnzyme
10% (v/v): Mea hoʻoheheʻe organik (1)
100 mM: Palena phosphate (pH7.0)
0.2 mg/ml: NAD+
100 mM: ʻAmonium formate
10 mg/ml: ʻO ka formate dehydrogenase (FDH)
E hoʻomoʻa i nā hopena ma kahi mea lūlū mahana mau (no ka laʻana, 30oC, 150rpm) no 24 ~ 48 mau hola. E centrifuge i kēlā me kēia hui e hoʻoulu i ka protein, hiki ke hoʻohana ʻia ka supernatant no ka nānā ʻana o TLC, HPLC a i ʻole GC.
Nānā (1): He koho ka mea hoʻoheheʻe organik (e like me DMSO, methanol, acetonitrile, IPA, a pēlā aku), hoʻohana ʻia no nā substrates insoluble.
