Nitrile hydratase (NHT)
ʻO ka wehewehe pōkole:
E pili ana i nā ES-NHT
Nā ES-NHTs: he papa o ka metalloenzyme, e hoʻomaʻemaʻe i nā nitriles i loko o ka amide kumu.Hoʻohana nui ʻia lākou ʻaʻole wale no ka synthesis o nā amino acids, amides, carboxylic acids a me kā lākou derivatives, akā no ka hoʻomākaukau ʻana i nā lāʻau chiral.Aia he 24 mau ʻano nitrile hydratases i hoʻomohala ʻia e SyncoZymes (Helu e like me ES-NHT-101～ES-NHT-124).
ʻAno hoʻopā catalytic:
Mobile/Wechat/WhatsApp: +86-13681683526
E-Mail:lchen@syncozymes.com
| Nā Enzymes | Code Huahana | Hōʻike |
| ʻO ka pauka Enzyme | ES-NHT-101~ ES-NHT-124 | he set o 24 Nitrile Hydratases, 50 mg kēlā me kēia 24 mau mea * 50mg / mea, a i ʻole ka nui ʻē aʻe |
| Mea Hoʻomaʻamaʻa (SynKit) | ES-NHT-2400 | he set o 24 Nnitrile Hydratases, 1 mg i kēlā me kēia 24 mau mea * 1mg / mea |
★ High substrate specificity.
★ Ke koho kiral ikaika.
★ hoʻololi kiʻekiʻe.
★ Liʻiliʻi nā huahana.
★ Kūlana haʻahaʻa haʻahaʻa.
★ Pili kaiapuni.
➢ ʻO ka mea maʻamau, pono e komo i loko o ka ʻōnaehana hoʻololi ke pani, ka solution buffer (pH hopena maikaʻi loa) a me ES-NHT.
➢ Hiki ke ho'āʻo ʻia nā ES-NHT a pau ma ka ʻōnaehana pane ma luna a i ʻole me ka NHT Screening Kit (SynKit NHT).
➢ Pono e aʻo pākahi ʻia nā ʻano ES-NHT a pau e pili ana i nā ʻano hopena maikaʻi loa.
➢ Hiki ke kaohi i ka hana a ES-NHT.Eia nō naʻe, hiki ke hoʻokuʻu ʻia ka inhibition me ka hoʻohui pū ʻana o ka substrate, ka hui pū ʻana me nā amidases a i ʻole ka unuhi ʻana i nā huahana.
Ka Laʻana 1 (hui pū me ka amidase)(1):
Laʻana 2(2):
E mālama i 2 mau makahiki ma lalo o -20 ℃.
Mai launa pū me nā kūlana koʻikoʻi e like me: ka wela kiʻekiʻe, ka pH kiʻekiʻe/haʻahaʻa a me ka mea hoʻoheheʻe organik kiʻekiʻe.
1 Vojtech V, Ludmila M, Alicja BV, a me tal.J Mol Catal B-enzym, 2011, 71: 51-55.
2 Gary W. B, Thomas G, Christopher B. M, e tal.Tetrahedron lett, 2010, 51: 1639-1641.
