Nitrile reductase (NRED)
ʻO ka wehewehe pōkole:
E pili ana i ka Nitrile reductase
Aia 3 ʻano huahana enzyme NRED (Helu e like me ES-NRED101~ ES-NRED103) i hoʻomohala ʻia e SyncoZymes.Hiki i ka NRED ke ho'ēmi i ka hui nitrile i amine.I ke kaʻina hana catalysis, pono ʻo NADPH ma ke ʻano he hydrogen transporter.
Nitrile reductasecatalytic reaction type
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E-mail:lchen@syncozymes.com
| Nā Enzymes | Code Huahana | Hōʻike |
| Hōʻike | ES- NRED -101~ ES- NRED -103 | kahi hoʻonohonoho o 3 Nitrile Reductase, 50 mg kēlā me kēia 3 mau mea * 50mg / mea, a i ʻole ka nui ʻē aʻe |
Hiki ke hoʻohana ʻia kēia ʻōnaehana pane no ke kuhikuhi ʻana:
1-10 mg/ml Lapaʻau
10 mg/ml Enzyme
10% (v/v) Mea hoʻoheheʻe kino (1)
100 mM Paʻa Phosphate (pH7.0)
0.2 mg/ml NADP+
10 mg/ml Glucose
2 mg/ml Glucose dehydrogenase (GDH)
Hoʻomoʻa i nā hopena ma kahi haʻalulu wela mau (no ka laʻana, 30oC, 150rpm) i loko no 24-48 mau hola.ʻO Centrifuge i kēlā me kēia hui e hoʻoheheʻe i ka protein, hiki ke hoʻohana ʻia ka supernatant no ka TLC, HPLC a i ʻole GC loiloi.
Nānā (1): ʻO ka solvent organik (e like me DMSO, methanol, acetonitrile, IPA, etc.) he koho, hoʻohana ʻia no nā substrates insoluble.
