Alcohol oxidase (AOX)
ʻO ka wehewehe pōkole:
E pili ana i ka waiʻona oxidase
ES-AOXs: Hiki i kēia mau enzymes ke hoʻopololei i ka hoʻoheheʻe ʻia o nā momona momona a i ʻole nā aryl-alcohols e hana i nā aldehydes.Hoʻohana lākou i ka oxygen molekula akā ʻaʻole koi i kahi cofactor i hoʻohui ʻia i waho, a hana ʻia ka hydrogen peroxide.
Aia he 6 mau ʻano huahana oxidases alcohol (Helu e like me ES-AOX-101~ES-AOX-106) i hoʻomohala ʻia e SyncoZymes.Makemake ʻo ES-AOX101 a me ES-AOX102 i nā substrate waiʻona aliphatic, ʻo ES-AOX103~ ES-AOX105 makemake i nā substrate waiʻona ʻala, a ʻo ES-AOX106 ka cholesterol oxidase.He mea hana pono ka SZ-AOX no ka hoʻopaʻa ʻana i nā ʻona momona a i ʻole nā aryl-alcohols e hana i nā aldehydes.
ʻAno hoʻopā catalytic:
Mobile/Wechat/WhatsApp: +86-13681683526
E-mail:lchen@syncozymes.com
| Nā Enzymes | Code Huahana | Hōʻike |
| ʻO ka pauka Enzyme | ES-AOX-101~ ES-AOX-105 | he pūʻulu o 5 alcohol oxidases, 50 mg kēlā me kēia 5 mau mea * 50mg / mea, a i ʻole ka nui ʻē aʻe |
| Mea Hoʻomaʻamaʻa (SynKit) | ES-AOX-500 | he pūʻulu o 5 alcohol oxidases, 50 mg kēlā me kēia 5 mau mea * 50mg / mea, a i ʻole ka nui ʻē aʻe |
★ High substrate specificity.
★ hoʻololi kiʻekiʻe.
★ Liʻiliʻi nā huahana.
★ Nā kūlana hoʻonā haʻahaʻa.
★ Pili kaiapuni.
➢ ʻO ka mea maʻamau, pono e hoʻokomo ʻia ka ʻōnaehana hoʻololi i ka substrate, ka solution buffer a me ES-AOX, a aia ka oxygen.
➢ ʻO nā ʻano ES-AOX āpau e pili ana i nā ʻano ʻano hopena maikaʻi loa, hiki ke aʻo ʻia pākahi.
➢ Hiki ke kaohi i ka hana a ES-AOX.Eia naʻe, hiki ke hoʻokuʻu ʻia ka inhibition me ka hoʻohui pū ʻana o ka substrate.
➢ ʻO ka hōʻiliʻili ʻana o H2O2i loko o ka ʻōnaehana e alakaʻi i ka enzyme inactivation, hiki ke hoʻoponopono maikaʻi ʻia e ka hoʻohana ʻana i ka catalase.
Laʻana 1(Oxidation of aryl-alcohols)(1):
Laʻana 2(Oxidation of fatty alcohols)(2):
E mālama i 2 mau makahiki ma lalo o -20 ℃.
Mai hoʻopili i nā kūlana koʻikoʻi e like me: ka wela kiʻekiʻe, ka pH kiʻekiʻe/haʻahaʻa a me ka mea hoʻoheheʻe kūlohelohe kiʻekiʻe.
1. Benen J AE, Sa' nchez-Torres P, Wagemaker M JM, e tal.J Biol Chem, 1998, 273(14): 7865-7872.
2. Mauersberger S, Drechsler H, Oehme G, e tal.Appl Microbiol Biot, 1992, 37: 66-73.
