Nitrilase (NIT)
ʻO ka wehewehe pōkole:
No Nitrilase
ES-NITs: Hoʻololi pololei i ka hui cyanide i ka hui carboxyl, e hoʻomākaukau ai i ka waikawa carboxylic, i hoʻohana nui ʻia i ka lāʻau lapaʻau, ka mālama ʻana i ke kaiapuni, ʻoihana kemika, mea hoʻohui meaʻai, textile, hana mahiʻai a me nā māla ʻē aʻe.
He 40 mau ʻano huahana Nitrilase(NIT) (Helu e like me ES-NIT-101~ES-NIT-140) i hoʻomohala ʻia e SyncoZymes.He mea hana pono ka ES-NIT no ka hoʻopili ʻana i ka synthesis o nā waikawa carboxylic chiral ma o ka regio- a me stereoselective hydrolysis o kahi ākea o nā aliphatic a me nā nitriles aromatic.
ʻAno hoʻopā catalytic:
Mobile/Wechat/WhatsApp: +86-13681683526
E-mail:lchen@syncozymes.com
| Nā Enzymes | Code Huahana | Hōʻike |
| ʻO ka pauka Enzyme | ES-NIT-101~ ES-NIT-140 | he set o 40 Nitrilase, 50 mg i kēlā me kēia 40 mau mea * 50mg / mea, a i ʻole ka nui ʻē aʻe |
| Mea Hoʻomaʻamaʻa (SynKit) | ES-NIT-4000 | kahi hoʻonohonoho o 40 Nitrilase, 1 mg i kēlā me kēia 40 mau mea * 1mg / mea |
★ High substrate specificity.
★ hoʻololi kiʻekiʻe.
★ Liʻiliʻi nā huahana.
★ Nā kūlana hoʻonā haʻahaʻa.
★ Pili kaiapuni.
➢ ʻO ka mea maʻamau, pono e hoʻokomo i ka ʻōnaehana pane ʻana i ka substrate, ka solution buffer (pH hopena maikaʻi loa) a me ES-NIT.
➢ Hiki ke hoʻāʻo ʻia nā ES-NIT a pau ma ka ʻōnaehana pane ma luna a i ʻole me ka NIT Screening Kit (SynKit NIT).
➢ Pono e aʻo pākahi ʻia nā ʻano ES-NIT a pau e pili ana i nā ʻano hopena maikaʻi loa.
➢ Hiki ke kaohi i ka hana a ES-NIT.Eia naʻe, hiki ke hoʻokuʻu ʻia ka inhibition me ka hoʻohui pū ʻana o ka substrate.
Laʻana 1(Synthesis of nicotinic acid)(1):
Laʻana 2(Synthesis of (R)-(-)-mandelic acid)(2):
Laʻana 3(Synthesis of baclofen)(3):
Laʻana 4(Synthesis of glycolic acid)(4):
E mālama i 2 mau makahiki ma lalo o -20 ℃.
Mai hoʻopili i nā kūlana koʻikoʻi e like me: ka wela kiʻekiʻe, ka pH kiʻekiʻe/haʻahaʻa a me ka mea hoʻoheheʻe kūlohelohe kiʻekiʻe.
1 Mathew CD, Nagasawa T, Kobayashi M, e tal.Appl.Kaiapuni.Microbiol, 1988, 54(4): 1030.
2 Yamamoto K, Oishi K, Fujimatsu I, e tal.Appl.Kaiapuni.Microbiol, 1991, 57(10): 3028.
3 Xu MZ, Ren J, Gong JS, a me ka tal.Chin.J. Biotechnol, 2013, 29(1): 31.
4 Wu S, Fogiel AJ, Petrillo KL, e tal.Biotechnol.Bioeng., 2008, 99(3): 717.
