ʻAmine Dehydrogenase (AmDH)
Wehewehe Pōkole:
E pili ana i ka Amine Dehydrogenase
ʻO ES-AmDH (Amine Dehydrogenase): kahi enzyme hiki ke hoʻoulu i ka ammoniation o ka hui carbonyl a hoʻemi iā ia i ka hui amino. He coenzyme ia e hilinaʻi ana i ka NADH, a hana ʻo NADH ma ke ʻano he mea hoʻoili hydrogen. Aia he 4 ʻano o nā huahana enzyme AmDH (Helu e like me ES-AmDH101～ES-AmDH104) i hoʻomohala ʻia e SyncoZymes, hiki ke hoʻohana ʻia i ka regioselective a i ʻole stereoselective reductive amination o nā aldehydes a me nā ketones.
ʻAno hana catalytic:
Kelepona Paʻalima/Wechat/WhatsApp: +86-13681683526
Leka uila:lchen@syncozymes.com
★ Kiʻekiʻe ke ʻano kikoʻī o ka substrate.
★ Koho chiral ikaika.
★ Hoʻololi kiʻekiʻe.
★ Emi nā huahana ʻaoʻao.
★ Nā kūlana pane iki.
★ Mālama i ke kaiapuni.
➢ ʻO ka maʻamau, pono e hoʻokomo pū ka ʻōnaehana hana i ka substrate, ka hopena buffer, ka enzyme, ka coenzyme a me ka ʻōnaehana hana hou ʻana o ka coenzyme (e laʻa me ka glucose a me ka glucose dehydrogenase).
➢ Pono e hoʻohui hope ʻia ʻo AmDH i loko o ka ʻōnaehana hana, ma hope o ka hoʻoponopono ʻia ʻana o ka pH a me ka mahana i ke kūlana hana.
Laʻana 1 (Hana ʻia o nā amine chiral e pili ana ma o ka hoʻemi ʻana o nā alkyl aryl ketones)(1):
E mālama no 2 mau makahiki ma lalo o -20℃.
Mai hoʻopili aku me nā kūlana koʻikoʻi e like me: ke kiʻekiʻe o ka mahana, ka pH kiʻekiʻe/haʻahaʻa a me ka mea hoʻoheheʻe organik kiʻekiʻe.
1. Kong W, Liu Y, Huang C, et al. ʻO Angewandte Chemie International Edition, 2022: e202202264.
