ʻO ka reductase waikawa carboxylic (CAR)
Wehewehe Pōkole:
E pili ana i ka reductase waikawa Carboxylic
ES-CAR (Carboxylic acid reductase): hoʻoulu i ka hōʻemi ʻana o ka hui carboxyl i ka hui aldehyde. I ke kaʻina hana catalytic, pono ka hoʻoulu ʻana o ATP a me ka coenzyme NADPH ma ke ʻano he mau mea lawe hydrogen. Aia he 2 ʻano o nā huahana enzyme CAR (Helu e like me ES-CAR101 ~ ES-CAR-102) i hoʻomohala ʻia e SyncoZymes.
ʻAno hana catalytic:
Kelepona Paʻalima/Wechat/WhatsApp: +86-13681683526
Leka uila:lchen@syncozymes.com
| Nā ʻenzyme | Kōmike Huahana | Nā kikoʻī |
| Pahu Nānā (SynKit) | ES-CAR-101-ES-CAR-102 | he set o 2 Ketoreductases, 1 mg kēlā me kēia 2 mau mea * 1mg / mea |
★ Kiʻekiʻe ke ʻano kikoʻī o ka substrate.
★ Koho chiral ikaika.
★ Hoʻololi kiʻekiʻe.
★ Emi nā huahana ʻaoʻao.
★ Nā kūlana pane iki.
★ Mālama i ke kaiapuni.
➢ ʻO ka maʻamau, pono e hoʻokomo pū ka ʻōnaehana hopena i ka substrate, ka hopena buffer, ka enzyme, coenzyme, ATP.
➢ Pono e hoʻohui hope ʻia ʻo CAR i loko o ka ʻōnaehana hana, ma hope o ka hoʻoponopono ʻia ʻana o ka pH a me ka mahana i ke kūlana hana.
Laʻana 1 (Hana ʻia ʻana o ka waiʻona cinnamyl mai ka phenylalanine)(1):
E mālama no 2 mau makahiki ma lalo o -20℃.
Mai hoʻopili aku me nā kūlana koʻikoʻi e like me: ke kiʻekiʻe o ka mahana, ka pH kiʻekiʻe/haʻahaʻa a me ka mea hoʻoheheʻe organik kiʻekiʻe.
1. Zhang, Chen, et al. Nā hale hana kelepona microbial 19.1 (2020): 1-10.
