Nucloside phosphoryalse (NP)

ʻO ka wehewehe pōkole:

E pili ana i ka nucleoside phosphoryalse

Aia 3 ʻano huahana enzyme NP (Helu e like me ES-NP-101~ ES-NP-103) i hoʻomohala ʻia e SyncoZymes.ʻO ES-NP-101 ka purine nucleoside phosphorylase, ES-NP-102 a me ES-NP-103 he pyrimidine nucleoside phosphorylase.Hiki i ka Nucleosidephosphorylase ke hoʻoheheʻe i nā nucleosides i nā kumu a me ka pentose phosphate.Hiki ke hoʻokaʻawale ʻia ka Nucleoside phosphorylase i purine nucleoside phosphorylase a me pyrimidine nucleoside phosphorylase e like me ka makemake o nā kumu nucleoside.Hiki i ka purine nucleoside phosphorylase ke hoʻololi i ka adenosine i adenine, inosine i hypoxanthine, guanosine i guanine, a hana i ka ribose phosphate i ka manawa like.Hiki i ka Pyrimidine nucleoside phosphorylase ke hoʻololi i ka uridine i ka uracil a hana i ka ribose phosphate.

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Huahana Huahana

Nucloside phosphoryalse reaction type:

ʻIkepili Huahana:

Nā Enzymes	Code Huahana	Hōʻike
ʻO ka pauka Enzyme	ES-NP-101~ ES-NP-103	kahi hoʻonohonoho o 3 Nuclosidephosphoryalse, 50 mg kēlā me kēia 3 mau mea * 50mg / mea, a i ʻole ka nui ʻē aʻe

Nucloside phosphoryalse screening reactions:

Hiki ke hoʻohana ʻia kēia ʻōnaehana pane no ke kuhikuhi ʻana:
1-10 mg/ml Lapaʻau
10 mg/ml Enzyme
100 mM Paʻa Phosphate (pH7.0)
Hoʻomoʻa i nā hopena ma kahi haʻalulu wela mau (no ka laʻana, 30oC, 150rpm) i loko no 12~24 hola.ʻO Centrifuge i kēlā me kēia hui e hoʻoheheʻe i ka protein, hiki ke hoʻohana ʻia ka supernatant no ka TLC, HPLC a i ʻole GC analysis.