Kiʻi i hōʻike ʻia ʻo Nucloside phosphoryalse (NP)

ʻO ka phosphoryalse nucloside (NP)

Wehewehe Pōkole:

E pili ana i ka phosphoryalse nucleoside

Aia he 3 ʻano o nā huahana enzyme NP (Helu e like me ES-NP-101 ~ ES-NP-103) i hoʻomohala ʻia e SyncoZymes. ʻO ES-NP-101 ka purine nucleoside phosphorylase, ʻo ES-NP-102 a me ES-NP-103 ka pyrimidine nucleoside phosphorylase. Hiki i ka Nucleosidephosphorylase ke hoʻokaʻawale i nā nucleosides i nā kumu a me ka pentose phosphate. Hiki ke hoʻokaʻawale ʻia ka Nucleoside phosphorylase i ka purine nucleoside phosphorylase a me ka pyrimidine nucleoside phosphorylase e like me ka makemake o nā kumu nucleoside. Hiki i ka Purine nucleoside phosphorylase ke hoʻololi i ka adenosine i adenine, inosine i hypoxanthine, guanosine i guanine, a hana i ka ribose phosphate i ka manawa like. Hiki i ka Pyrimidine nucleoside phosphorylase ke hoʻololi i ka uridine i uracil a hana i ka ribose phosphate.

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Nā kikoʻī huahana

Nā Lepili Huahana

ʻAno hana phosphoryalse nucloside:

ʻIke Huahana:

Nā ʻenzyme	Kōmike Huahana	Nā kikoʻī
Pauka ʻenzyme	ES-NP-101~ ES-NP-103	he set o 3 Nuclosidephosphoryalse, 50 mg kēlā me kēia 3 mau mea * 50mg / mea, a i ʻole ka nui ʻē aʻe

Ke kaʻina hana hoʻāʻo ʻana o ka nucloside phosphoryalse:

Hiki ke hoʻohana ʻia ka ʻōnaehana hana ma lalo nei no ka ʻike:
1-10 mg/ml Pākuʻi
10 mg/ml ʻEnzyme
100 mM Phosphate buffer (pH7.0)
E hoʻomoʻa i nā hopena ma kahi mea lūlū mahana mau (no ka laʻana, 30oC, 150rpm) no 12 ~ 24 mau hola. E centrifuge i kēlā me kēia hui e hoʻoulu i ka protein, hiki ke hoʻohana ʻia ka supernatant no ka nānā ʻana o TLC, HPLC a i ʻole GC.