ʻO ka Transaminase (ATA)
Nā ʻenzymes: He mau mea hoʻoulu olaola macromolecular, ʻo ka hapa nui o nā enzymes he mau protein.
Nā Transaminases: He papa o nā enzymes e hoʻoulu ai i ka hoʻoili ʻana o ka amino ma waena o nā waikawa amino a me nā waikawa keto. ʻO nā Transaminases nā enzymes olaola koʻikoʻi i ka synthesis asymmetric a me ka hoʻonā racemic o nā amines chiral.
Hiki ke hoʻokaʻawale ʻia ka Aminotransferase i ʻehā mau papa e like me ke kaʻina a me ke ʻano: Ⅰ, Ⅱ, Ⅲ a me Ⅳ. No nā ω-aminotransferases nā transaminases papa II, hoʻohana pinepine ʻia i ka hoʻomākaukau ʻana i nā amine chiral a me nā waikawa amino kūlohelohe ʻole, e like me nā waikawa β-amino.
ω-aminotransferases: I ka hapanui o nā hihia, pili ka ω-transaminase i kahi papa o nā enzymes, nāna e hoʻoili i ka ammonia catalytic me ka ʻole o ka α-amino acid ma ke ʻano he substrate a huahana paha.
ʻAno hana hoʻouluulu:
| Nā ʻenzyme | Kōmike Huahana | Kōmike Huahana |
| Pauka ʻenzyme | ES-ATA-101~ ES-ATA-165 | he hoʻonohonoho o 65 ω-Transaminases, 50 mg kēlā me kēia 65 mau mea * 50mg / mea, a i ʻole ka nui ʻē aʻe |
| Pahu Nānā (SynKit) | ES-ATA-6500 | he hoʻonohonoho o 65 ω-Transaminases, 1 mg kēlā me kēia 65 mau mea * 1mg / mea |
★ Kiʻekiʻe ke ʻano kikoʻī o ka substrate.
★ Koho chiral ikaika.
★ Ka pono hoʻololi kiʻekiʻe.
★ Emi nā huahana ʻaoʻao.
★ Nā kūlana pane iki.
★ Mālama i ke kaiapuni.
➢ Pono e hoʻokō ʻia ka nānā ʻana o nā enzyme no nā substrates kikoʻī ma muli o ke ʻano kikoʻī o ka substrate, a loaʻa i kahi enzyme e hoʻoulu ai i ka substrate i manaʻo ʻia me ka hopena catalytic maikaʻi loa.
➢ Mai hoʻopā aku me nā kūlana koʻikoʻi e like me: ke kiʻekiʻe o ka mahana, ka pH kiʻekiʻe/haʻahaʻa a me ka mea hoʻoheheʻe organik me ka nui kiʻekiʻe.
➢ ʻO ka maʻamau, pono i ka ʻōnaehana hana ke komo pū me ka substrate, ka hopena buffer, ka mea hāʻawi amino (e like me nā waikawa amino a me 1-phenyl ethylamine) a i ʻole ka mea loaʻa (e like me nā waikawa keto), coenzyme (PLP), cosolvent (e like me DMSO).
➢ Pono e hoʻohui hope ʻia ʻo ATA i loko o ka ʻōnaehana hana, ma hope o ka hoʻoponopono ʻia ʻana o ka pH a me ka mahana i ke kūlana hana.
➢ Loaʻa i nā ʻano ATA āpau nā kūlana hopena kūpono like ʻole, no laila pono e aʻo hou ʻia kēlā me kēia.
Laʻana 1 (hana ʻia ʻana o Sitagliptin, hana ʻia ʻana asymmetric)(1):
Laʻana 2 (Mexiletine, Hoʻohui ʻia o ka hoʻonā kinetic me ka synthesis asymmetric)(2):
1 Savile CK, Janey JM, Mundorff EC, et al. ʻEpekema, 2010, 329(16), 305-309.
2 Koszelewski D, Pressnitz D, Clay D, et al. Nā leka kino, 2009,11(21):4810-4812.
