ʻO ka Benzaldehyde lyase (BAL)
Wehewehe Pōkole:
E pili ana i ka Benzaldehyde lyase
ʻO ES-BAL (Benzaldehyde lyase): kahi ʻano enzyme e hoʻoulu ai i ka hydroxymethylation o nā aldehydes aromatic a me aliphatic me ka formaldehyde e hana i ka α-Hydroxymethyl ketone like. ʻO BAL ka enzyme hilinaʻi thiamine diphosphate (ThDP). Hoʻokahi wale nō ʻano huahana enzyme BAL (Helu e like me ES-BAL101) i hoʻomohala ʻia e SyncoZymes, hiki ke hoʻohana ʻia no ka hydroxymethylation o nā aldehydes aromatic a me aliphatic.
ʻAno hana catalytic:
Kelepona Paʻalima/Wechat/WhatsApp: +86-13681683526
Leka uila:lchen@syncozymes.com
★ Kiʻekiʻe ke ʻano kikoʻī o ka substrate.
★ Koho chiral ikaika.
★ Hoʻololi kiʻekiʻe.
★ Emi nā huahana ʻaoʻao.
★ Nā kūlana pane iki.
★ Mālama i ke kaiapuni.
➢ ʻO ka maʻamau, pono e hoʻokomo pū ka ʻōnaehana hana i ka substrate, ka hopena buffer, ka enzyme, ThDP, Mg2+.
➢ Pono e hoʻohui hope ʻia ka BAL i loko o ka ʻōnaehana hana, ma hope o ka hoʻoponopono ʻia ʻana o ka pH a me ka mahana i ke kūlana hana.
Laʻana 1 (Hana ʻia o nā waiʻona amino 1,2 i hoʻololi ʻia e N mai nā aldehydes)(1):
E mālama no 2 mau makahiki ma lalo o -20℃.
Mai hoʻopili aku me nā kūlana koʻikoʻi e like me: ke kiʻekiʻe o ka mahana, ka pH kiʻekiʻe/haʻahaʻa a me ka mea hoʻoheheʻe organik kiʻekiʻe.
1. Li Y, Hu N, Xu Z, et al. Angewandte Chemie International Edition, 2022, 61(17): e202116344.
