Benzaldehyde lyase (BAL)
★ High substrate specificity.
★ Ke koho kiral ikaika.
★ hoʻololi kiʻekiʻe.
★ Liʻiliʻi nā huahana.
★ Kūlana haʻahaʻa haʻahaʻa.
★ Pili kaiapuni.
➢ ʻO ka mea maʻamau, pono e hoʻokomo i ka ʻōnaehana pane ʻana i kahi substrate, solution buffer, enzyme, ThDP, Mg2+.
➢ Pono e hoʻohui hope ʻia ka BAL i loko o ka ʻōnaehana pane, ma hope o ka hoʻoponopono ʻana o ka pH a me ka mahana i ke ʻano o ka hopena.
Laʻana 1(Synthesis of N-substituted 1,2 amino alcohols from aldehydes)(1):
E mālama i 2 mau makahiki ma lalo o -20 ℃.
Mai launa pū me nā kūlana koʻikoʻi e like me: ka wela kiʻekiʻe, ka pH kiʻekiʻe/haʻahaʻa a me ka mea hoʻoheheʻe organik kiʻekiʻe.
1. Li Y, Hu N, Xu Z, et al.Angewandte Chemie International Edition, 2022, 61(17): e202116344.
E kākau i kāu leka ma aneʻi a hoʻouna mai iā mākou