ʻO ka Cyclohexanone monooxygenase (CHMO)
Wehewehe Pōkole:
E pili ana i ka Cyclohexanone monooxygenase
ʻO ES-CHMO (Cyclohexanone monooxygenase): kahi Baeyer Villiger monooxygenase (BVMO) me ka cyclohexanone ma ke ʻano he substrate, hiki ke hoʻololi i ka cyclohexanone i cyclohexanol me ka hoʻohana ʻana i ka oxygen.
He ʻano enzyme ia e hilinaʻi ana i ka flavin. Hoʻemi ʻia ka flavin i oxidized ʻia i flavin ma o ke kaʻina hana o ka hoʻemi ʻana i ka coenzyme II (NADPH) no ka oxidation aʻe. No laila, pono ka cofactor NADPH i ka kaʻina hana flavin. Hoʻokahi wale nō ʻano huahana enzyme CHMO (Helu e like me ES-CHMO101) i hoʻomohala ʻia e SyncoZymes, hiki ke hoʻohana ʻia e oxidize i ka cyclohexanone i cyclohexanone.
ʻAno hana catalytic:
Kelepona Paʻalima/Wechat/WhatsApp: +86-13681683526
Leka uila:lchen@syncozymes.com
| Nā ʻenzyme | Kōmike Huahana | Nā kikoʻī |
| Pahu Nānā (SynKit) | ES-CHMO-101 | he set o 1 Ketoreductases, 1 mg kēlā me kēia 1 mau mea * 1mg / mea |
★ Kiʻekiʻe ke ʻano kikoʻī o ka substrate.
★ Koho chiral ikaika.
★ Hoʻololi kiʻekiʻe.
★ Emi nā huahana ʻaoʻao.
★ Nā kūlana pane iki.
★ Mālama i ke kaiapuni.
➢ ʻO ka maʻamau, pono e hoʻokomo pū ka ʻōnaehana hana i ka substrate, ka hopena buffer, ka enzyme, ka coenzyme a me ka ʻōnaehana hana hou ʻana o ka coenzyme (e laʻa me ka glucose a me ka glucose dehydrogenase).
➢ Pono e hoʻohui hope ʻia ʻo CHMO i loko o ka ʻōnaehana hana, ma hope o ka hoʻoponopono ʻia ʻana o ka pH a me ka mahana i ke kūlana hana.
Laʻana 1 (Hana ʻia ʻana o ka cyclohexanol mai ka cyclohexanol)(1):
E mālama no 2 mau makahiki ma lalo o -20℃.
Mai hoʻopili aku me nā kūlana koʻikoʻi e like me: ke kiʻekiʻe o ka mahana, ka pH kiʻekiʻe/haʻahaʻa a me ka mea hoʻoheheʻe organik kiʻekiʻe.
1. Friso, Marco, Appl Microbiol Biotechnol, 2017, 101(20): 7557-7565
