Cyclohexanone monooxygenase (CHMO)
ʻO ka wehewehe pōkole:
E pili ana iā Cyclohexanone monooxygenase
ES-CHMO (Cyclohexanone monooxygenase): kahi Baeyer Villiger monooxygenase (BVMO) me ka cyclohexanone ma ke ʻano he substrate, hiki ke hoʻokahe i ka cyclohexanone i cyclohexanol me ka hoʻohana ʻana i ka oxygen.
He ʻano enzyme pili flavin.Hoʻemi ʻia ka flavin oxidized i flavin ma o ka pōʻaiapuni o ka hoʻemi ʻana i ka coenzyme II (NADPH) no ka hoʻonā ʻana aʻe.No laila, pono ka cofactor NADPH i ka pōʻai flavin.Hoʻokahi wale nō ʻano huahana enzyme CHMO (Helu e like me ES-CHMO101) i hoʻomohala ʻia e SyncoZymes, hiki ke hoʻohana ʻia e oxidize cyclohexanone i cyclohexanone.
ʻAno hoʻopā catalytic:
Mobile/Wechat/WhatsApp: +86-13681683526
E-mail:lchen@syncozymes.com
| Nā Enzymes | Code Huahana | Hōʻike |
| Mea Hoʻomaʻamaʻa (SynKit) | ES-CHMO-101 | he set o 1 Ketoreductases, 1 mg i kēlā me kēia 1 mea * 1mg / mea |
★ High substrate specificity.
★ Ke koho kiral ikaika.
★ hoʻololi kiʻekiʻe.
★ Liʻiliʻi nā huahana.
★ Nā kūlana hoʻonā haʻahaʻa.
★ Pili kaiapuni.
➢ ʻO ka mea maʻamau, pono e hoʻokomo i ka ʻōnaehana hoʻololi i ka substrate, ka solution buffer, ka enzyme, ka coenzyme a me ka coenzyme regeneration system (e laʻa me ka glucose a me ka glucose dehydrogenase).
➢ Pono e hoʻohui hope ʻia ka CHMO i loko o ka ʻōnaehana pane, ma hope o ka hoʻololi ʻana o ka pH a me ka mahana i ke ʻano o ka hopena.
Ka Laʻana 1(Synthesis of cyclohexanol from cyclohexanol)(1):
E mālama i 2 mau makahiki ma lalo o -20 ℃.
Mai hoʻopili i nā kūlana koʻikoʻi e like me: ka wela kiʻekiʻe, ka pH kiʻekiʻe/haʻahaʻa a me ka mea hoʻoheheʻe kūlohelohe kiʻekiʻe.
1. Friso, Marco, Appl Microbiol Biotechnol, 2017, 101(20): 7557-7565
