ʻO ka D-Amino acid dehydrogenase (D-AADH)
Wehewehe Pōkole:
E pili ana i ka D-Amino acid dehydrogenase
ʻO ES-D-AADH (D-Amino acid dehydrogenase): kahi enzyme e hiki ke hoʻemi i ka 2-keto acid i loko o ka D-amino acid e pili ana. Pono ka hopena i ka NADP(H) a me ka mea hāʻawi ammonia inorganic (e like me ka ammonia a i ʻole ka ammonium ion). Aia he 17 ʻano o nā D-amino acid dehydrogenases (Helu e like me ES-DAADH-101 ~ ES-DAADH-117) i hoʻomohala ʻia e SyncoZymes.
ʻAno hana catalytic:
Kelepona Paʻalima/Wechat/WhatsApp: +86-13681683526
Leka uila:lchen@syncozymes.com
| Nā ʻenzyme | Kōmike Huahana | Nā kikoʻī |
| Pauka ʻenzyme | ES-D-AADH-101~ ES-D-AADH-117 | he hoʻonohonoho o 17 D-amino Acid Dehydrogenases, 50 mg kēlā me kēia 17 mau mea * 50mg / mea, a i ʻole ka nui ʻē aʻe |
| Pahu Nānā (SynKit) | ES-D-AADH-1700 | he hoʻonohonoho o 17 mau D-amino Acid Dehydrogenases, 1 mg kēlā me kēia 17 mau mea * 1mg / mea |
★ Kiʻekiʻe ke ʻano kikoʻī o ka substrate.
★ Koho chiral ikaika.
★ Hoʻololi kiʻekiʻe.
★ Emi nā huahana ʻaoʻao.
★ Nā kūlana pane iki.
★ Mālama i ke kaiapuni.
➢ ʻO ka maʻamau, pono e hoʻokomo pū ka ʻōnaehana hana i ka substrate, ka hopena buffer, ka enzyme, ka coenzyme a me ka ʻōnaehana hana hou ʻana o ka coenzyme (e laʻa me ka glucose a me ka glucose dehydrogenase).
➢ Pono e hoʻohui hope ʻia ʻo D-AADH i loko o ka ʻōnaehana hana, ma hope o ka hoʻoponopono ʻia ʻana o ka pH a me ka mahana i ke kūlana hana.
Laʻana 1 (Hoʻemi ʻana i nā waikawa α Ketoa i nā waikawa D-amino e pili ana)(1):
E mālama no 2 mau makahiki ma lalo o -20℃.
Mai hoʻopili aku me nā kūlana koʻikoʻi e like me: ke kiʻekiʻe o ka mahana, ka pH kiʻekiʻe/haʻahaʻa a me ka mea hoʻoheheʻe organik kiʻekiʻe.
1. ʻAkita H, Hayashi J, Sakuraba H, et al. Nā Palena ma Microbiology, 2018, 9: 1760.
