ʻEne reductase (ERED)
Hoʻouluulu nā ES-ERED i nā ʻano substrate like ʻole ma muli o ka laulā o nā substrates. Ma keʻano laulā, hiki ke hoʻemi maʻalahi ʻia ka C=C o nā hui α, β-unsaturated me nā hui electron-absorbing (me ketone aldehyde, nā hui nitro, nā waikawa carboxylic, esters, anhydride, lactones, imines, etc.) e nā ES-ERED, akā ʻaʻole nā pilina pālua i hoʻāla ʻole ʻia.
Aia he 46 ʻano huahana enzyme ERED (Helu e like me ES-ERED-101~ES-ERED-146) i hoʻomohala ʻia e SyncoZymes.
ʻAno hana hoʻouluulu:
| Nā ʻenzyme | Kōmike Huahana | Nā kikoʻī |
| Pauka ʻenzyme | ES-ERED-101~ ES-ERED-146 | he seti o 46 Ene Reductases, 50 mg kēlā me kēia 46 mau mea * 50mg / mea, a i ʻole ka nui ʻē aʻe |
| Pahu Nānā (SynKit) | ES-ERED-4600 | he seti o 46 Ene Reductases, 50 mg kēlā me kēia 46 mau mea * 50mg / mea, a i ʻole ka nui ʻē aʻe |
★ Kiʻekiʻe ke ʻano kikoʻī o ka substrate.
★ Koho chiral ikaika.
★ Hoʻololi kiʻekiʻe.
★ Emi nā huahana ʻaoʻao.
★ Nā kūlana pane iki.
★ Mālama i ke kaiapuni.
★ Palekana kiʻekiʻe.
➢ ʻO ka maʻamau, pono i ka ʻōnaehana hana ke komo pū me ka substrate, ka hopena buffer (pH hopena maikaʻi loa), nā coenzymes (NAD(H) a i ʻole NADP(H)), ka ʻōnaehana hana hou ʻana o ka coenzyme (e laʻa me ka glucose a me ka glucose dehydrogenase) a me ES-ERED.
➢ Hiki ke hoʻāʻo ʻia nā ES-ERED āpau ma ka ʻōnaehana hopena ma luna a i ʻole me ka ERED Screening Kit (SynKit ERED).
➢ Pono e aʻo pākahi ʻia nā ʻano ES-ERED āpau e pili ana i nā kūlana hopena kūpono like ʻole.
➢ Hiki i ka substrate a i ʻole ka huahana kiʻekiʻe ke kāohi i ka hana a ES-ERED. Eia nō naʻe, hiki ke hoʻomaha ʻia ke kāohi ʻana ma o ka hoʻohui ʻana o ka substrate.
Laʻana 1 (α,β-unsaturated aldehydes a i ʻole ketones)(1):
Laʻana 2 (α,β-unsaturated carboxylic acids a me kā lākou mau derivatives)(2):
E mālama no 2 mau makahiki ma lalo o -20℃.
Mai hoʻopili aku me nā kūlana koʻikoʻi e like me: ke kiʻekiʻe o ka mahana, ka pH kiʻekiʻe/haʻahaʻa a me ka mea hoʻoheheʻe organik kiʻekiʻe.
1. Lucídio C, Fardelone J, Augusto R, e tal. J.Mol.Catal.B: Enzym., 2004, 29: 41-45.
2. Stueckler C, Hall M, Ehammer H, a me tal. .Org.Lett, 2007, 9(26): 5409-5411.
