ʻImine reductase (IRED)
Wehewehe Pōkole:
E pili ana i ka Imine reductase
ES-IRED: He papa o nā enzymes e hoʻoulu ai i ka hōʻemi ʻana o ka pilina C=N i ka pilina CN. No ka enzyme redox ia, me ka coenzyme NADPH no ka lawe ʻana i ka hydrogen. Wahi a ka makemake chiral o nā huahana, hiki ke hoʻokaʻawale ʻia iā R-IRED a me S-IRED. Ua hoʻomohala ʻo Syncozymes i 15 mau imine reductases (numbIRED ES-IRED-101-ES-IRED-115), hiki ke hoʻohana nui ʻia i ka hōʻemi regioselective a me stereoselective o nā imines e hoʻomākaukau i nā amines chiral.
ʻAno hana catalytic:
Kelepona Paʻalima/Wechat/WhatsApp: +86-13681683526
Leka uila:lchen@syncozymes.com
| Nā ʻenzyme | Kōmike Huahana | Nā kikoʻī |
| Pauka ʻenzyme | ES-IRED-101~ ES-IRED-114 | he seti o 14 Imine Reductase, 50 mg kēlā me kēia |
| Pahu Nānā Enzyme 96-Well | ES-IRED-1400 | he seti o 14 Imine Reductase, 1 mg kēlā me kēia |
★ Kiʻekiʻe ke ʻano kikoʻī o ka substrate.
★ Koho chiral ikaika.
★ Hoʻololi kiʻekiʻe.
★ Emi nā huahana ʻaoʻao.
★ Nā kūlana pane iki.
★ Mālama i ke kaiapuni.
➢ ʻO ka maʻamau, pono e hoʻokomo pū ka ʻōnaehana hana i ka substrate, ka hopena buffer, ka enzyme, ka coenzyme, a me ka ʻōnaehana hana hou ʻana o ka coenzyme.
➢ Pono e aʻo pākahi ʻia nā ʻano ES-IRED āpau e pili ana i nā kūlana hopena kūpono like ʻole.
➢ Hiki i ka substrate a i ʻole ka huahana kiʻekiʻe ke kāohi i ka hana a ES-IRED. Eia nō naʻe, hiki ke hoʻomaha ʻia ke kāohi ʻana ma o ka hoʻohui ʻana o ka substrate.
Laʻana 1 (Biosynthesis o chiral 2-methyl pyrrolidine)(1):
Laʻana 2 (Biosynthesis o ka amine lua)(2):
Laʻana 3 (Hoʻemi ʻia o nā Cyclic Imines)(3):
E mālama no 2 mau makahiki ma lalo o -20℃.
Mai hoʻopili aku me nā kūlana koʻikoʻi e like me: ke kiʻekiʻe o ka mahana, ka pH kiʻekiʻe/haʻahaʻa a me ka mea hoʻoheheʻe organik kiʻekiʻe.
1. Scheller PN, Fademrecht S, Hofelzer S, et al. ChemBioChem, 2014, 15, 2201-2204.
2. Wetzl D, Gand M, Ross A, et al. ChemCatChem, 2016, 8, 2023-2026.
3. Li H, Luan ZJ, Zheng GW, et al. ʻO Adv. Synth. Catal. 2015, 357, 1692-1696.
