ʻO ka Hydroaminase (HAM)
Wehewehe Pōkole:
E pili ana i ka Hydroaminase
Aia he 2 ʻano huahana enzyme HAM (Helu e like me ES-HAM-101 ~ ES-HAM-102) i hoʻomohala ʻia e SyncoZymes. Hiki i ka HAM ke hoʻoulu i ka ammoniation o ka waikawa enoic a i ʻole kāna mau derivatives e hana i nā waikawa amino chiral. Hiki ke hoʻohana ʻia ka HAM e synthesize i nā waikawa amino chiral (a i ʻole nā amino chiral) mai nā waikawa enoic (a i ʻole alkenes). Pono ka mea hāʻawi ammonia, e like me ka wai ammonia a i ʻole ka paʻakai ammonium.
ʻAno hana catalytic hydroaminase
Kelepona Paʻalima/Wechat/WhatsApp: +86-13681683526
Leka uila:lchen@syncozymes.com
| Nā ʻenzyme | Kōmike Huahana | Nā kikoʻī |
| Pauka ʻenzyme | ES-HAM-101~ ES-HAM-102 | he set o 2 Nuclosidephosphoryalse, 50 mg kēlā me kēia 2 mau mea * 50mg / mea, a i ʻole ka nui ʻē aʻe |
Hiki ke hoʻohana ʻia ka ʻōnaehana hana ma lalo nei no ka ʻike:
1-10 mg/ml: Pākuʻi
10 mg/ml: ʻEnzyme
10% (v/v): Mea hoʻoheheʻe organik (1)
10% (v/v): ʻAmonia
E hoʻoponopono i ka pH i 9.0 ma mua o ka hoʻohui ʻana i ka enzyme, a pono e hoʻohui hope ʻia ka enzyme. E hoʻomoʻa i nā hopena ma kahi mea lūlū mahana mau (no ka laʻana, 30oC, 150rpm) no 24 ~ 48 mau hola. E centrifuge i kēlā me kēia hui e hoʻoulu i ka protein, hiki ke hoʻohana ʻia ka supernatant no ka nānā ʻana o TLC, HPLC a i ʻole GC.
Nānā (1): He koho ka mea hoʻoheheʻe organik (e like me DMSO, methanol, acetonitrile, IPA, a pēlā aku), hoʻohana ʻia no nā substrates insoluble.
