Hydroaminase (HAM)
ʻO ka wehewehe pōkole:
E pili ana i ka Hydroaminase
Aia 2 ʻano huahana enzyme HAM (Helu e like me ES-HAM-101~ ES-HAM-102) i hoʻomohala ʻia e SyncoZymes.Hiki i ka HAM ke hoʻokaʻawale i ka ammoniation o ka waika enoic a i ʻole nā mea i loaʻa mai e hana i nā waikawa amino chiral.Hiki ke hoʻohana ʻia ka HAM e hoʻohui i nā ʻakika amino chiral (a i ʻole chiral amines) mai nā waikawa enoic (a i ʻole alkenes).Pono ka mea hāʻawi Ammonia, e like me ka wai amonia a i ʻole ka paʻakai ammonium.
Hydroaminase catalytic pane ʻano
Mobile/Wechat/WhatsApp: +86-13681683526
E-mail:lchen@syncozymes.com
| Nā Enzymes | Code Huahana | Hōʻike |
| ʻO ka pauka Enzyme | ES-HAM-101~ ES-HAM-102 | kahi hoʻonohonoho o 2 Nuclosidephosphoryalse, 50 mg kēlā me kēia 2 mau mea * 50mg / mea, a i ʻole ka nui ʻē aʻe |
Hiki ke hoʻohana ʻia kēia ʻōnaehana pane no ke kuhikuhi ʻana:
1-10 mg/ml: Pāpā
10 mg/ml: Enzyme
10% (v/v): Mea hoʻoheheʻe kino (1)
10% (v/v): ʻAmonia
E hoʻololi i ka pH i 9.0 ma mua o ka hoʻohui ʻana i ka enzyme, a pono e hoʻohui ʻia ka enzyme.Hoʻomoʻa i nā hopena ma kahi haʻalulu wela mau (no ka laʻana, 30oC, 150rpm) i loko no 24-48 mau hola.ʻO Centrifuge i kēlā me kēia hui e hoʻoheheʻe i ka protein, hiki ke hoʻohana ʻia ka supernatant no ka TLC, HPLC a i ʻole GC loiloi.
Nānā (1): ʻO ka solvent organik (e like me DMSO, methanol, acetonitrile, IPA, etc.) he koho, hoʻohana ʻia no nā substrates insoluble.
