ʻO ka Nitro reductase (NTR)
ʻAno hana catalytic:
ʻAno hana hoʻouluulu:
| Nā ʻenzyme | Kōmike Huahana | Nā kikoʻī |
| Pauka ʻenzyme | ES-NTR-101~ ES-NTR-112 | he seti o 12 Nitro Reductases, 50 mg kēlā me kēia 12 mau mea * 50mg / mea, a i ʻole ka nui ʻē aʻe |
| Pahu Nānā (SynKit) | ES-NTR-1200 | he seti o 12 Nitro Reductases, 1 mg kēlā me kēia 12 mau mea * 1mg / mea |
★ Ka laulā o ka substrate.
★ Hoʻololi kiʻekiʻe.
★ Emi nā huahana ʻaoʻao.
★ Nā kūlana pane iki.
★ Mālama i ke kaiapuni.
➢ ʻO ka maʻamau, pono i ka ʻōnaehana hana ke komo pū me ka substrate, ka hopena buffer (pH hopena maikaʻi loa), nā coenzymes (NAD(H) a i ʻole NADP(H)), ka ʻōnaehana hana hou ʻana o ka coenzyme (e laʻa me ka glucose a me ka glucose dehydrogenase) a me ES-NTR.
➢ Pono e aʻo pākahi ʻia nā ʻano ES-NTR āpau e pili ana i nā kūlana hopena kūpono like ʻole.
➢ Pono e hoʻohui hope ʻia ʻo ES-NTR i loko o ka ʻōnaehana hana me ka pH a me ka mahana hana kūpono loa.
Laʻana 1(1):
Laʻana 2(2):
E mālama no 2 mau makahiki ma lalo o -20℃.
Mai hoʻopili aku me nā kūlana koʻikoʻi e like me: ke kiʻekiʻe o ka mahana, ka pH kiʻekiʻe/haʻahaʻa a me ka mea hoʻoheheʻe organik kiʻekiʻe.
1 Dai RJ, Chen J, Lin J, e tal. J Hazard Marer, 2009, 170, 141–143.
2 Betancor L, Berne C, Luckarift H R., e tal. Kemi. Commun, 2006, 3640–3642.
