ʻO ka ʻona ʻokikene (AOX)
Wehewehe Pōkole:
E pili ana i ka waiʻona oxidase
ES-AOXs: Hiki i kēia mau enzymes ke hoʻoulu i ka oxidation o nā fatty alcohols a i ʻole aryl-alcohols e hana i nā aldehydes. Hoʻohana lākou i ka oxygen molekala akā ʻaʻole pono i kahi cofactor i hoʻohui ʻia mai waho, a hana ʻia ka hydrogen peroxide.
Aia he 6 ʻano huahana ʻona oxidases (Helu e like me ES-AOX-101~ES-AOX-106) i hoʻomohala ʻia e SyncoZymes. Makemake ʻo ES-AOX101 a me ES-AOX102 i nā substrates ʻona aliphatic, makemake ʻo ES-AOX103~ ES-AOX105 i nā substrates ʻona ʻala, a ʻo ES-AOX106 ka cholesterol oxidase. He mea hana pono ʻo SZ-AOX e hoʻoulu i ka oxidation o nā ʻona momona a i ʻole nā aryl-alcohols e hana i nā aldehydes.
ʻAno hana catalytic:
Kelepona Paʻalima/Wechat/WhatsApp: +86-13681683526
Leka uila:lchen@syncozymes.com
| Nā ʻenzyme | Kōmike Huahana | Nā kikoʻī |
| Pauka ʻenzyme | ES-AOX-101~ ES-AOX-105 | he hoʻonohonoho o 5 mau ʻona oxidases, 50 mg kēlā me kēia 5 mau mea * 50mg / mea, a i ʻole kekahi nui ʻē aʻe |
| Pahu Nānā (SynKit) | ES-AOX-500 | he hoʻonohonoho o 5 mau ʻona oxidases, 50 mg kēlā me kēia 5 mau mea * 50mg / mea, a i ʻole kekahi nui ʻē aʻe |
★ Kiʻekiʻe ke ʻano kikoʻī o ka substrate.
★ Hoʻololi kiʻekiʻe.
★ Emi nā huahana ʻaoʻao.
★ Nā kūlana pane iki.
★ Mālama i ke kaiapuni.
➢ ʻO ka maʻamau, pono e hoʻokomo pū ka ʻōnaehana hopena i ka substrate, ka hopena buffer a me ES-AOX, a pono hoʻi e loaʻa ka oxygen.
➢ Nā ʻano ES-AOX āpau e pili ana i nā kūlana hopena kūpono like ʻole, hiki ke aʻo ʻia pākahi.
➢ Hiki i ka substrate a i ʻole ka huahana kiʻekiʻe ke kāohi i ka hana a ES-AOX. Eia nō naʻe, hiki ke hoʻomaha ʻia ke kāohi ʻana ma o ka hoʻohui ʻana o ka substrate.
➢ Ka hōʻiliʻili ʻana o H2O2i loko o ka ʻōnaehana e alakaʻi ai i ka hoʻopau ʻana o ka enzyme, hiki ke hoʻoponopono pono ʻia ma ka hoʻohana ʻana i ka catalase.
Laʻana 1 (ʻOkika ʻana o nā aryl-alcohols)(1):
Laʻana 2 (ʻOkika ʻana o nā waiʻona momona)(2):
E mālama no 2 mau makahiki ma lalo o -20℃.
Mai hoʻopili aku me nā kūlana koʻikoʻi e like me: ke kiʻekiʻe o ka mahana, ka pH kiʻekiʻe/haʻahaʻa a me ka mea hoʻoheheʻe organik kiʻekiʻe.
1. Benen J AE, Sa' nchez-Torres P, Wagemaker M JM, e tal. J Biol Chem, 1998, 273(14): 7865-7872.
2. Mauersberger S, Drechsler H, Oehme G, e tal. Appl Microbiol Biot, 1992, 37: 66-73.
