ʻEsterase a me Lipase (PLE a me CALB)
Aia he 26 ʻano huahana enzyme PLE (Helu e like me ES-PLE-101～ES-PLE-126) i hoʻomohala ʻia e SyncoZymes. Hiki ke hoʻohana ʻia ʻo ES-PLE no ka hydrolysis o nā hui aliphatic a me ester, a i ʻole ka regioselective a me ka stereoselective resolution e synthesize i nā waikawa chiral a me kā lākou mau derivatives.
ʻAno hana catalytic:
★ Kiʻekiʻe ke ʻano kikoʻī o ka substrate.
★ Koho chiral ikaika.
★ Hoʻololi kiʻekiʻe.
★ Emi nā huahana ʻaoʻao.
★ Nā kūlana pane iki.
★ Mālama i ke kaiapuni.
➢ ʻO ka maʻamau, pono e hoʻokomo pū ka ʻōnaehana hopena i ka substrate, ka hopena buffer a me ka ES-PLE. Hana ʻia ka esterification o kekahi mau ES-PLE i ka pae organik.
➢ Pono e aʻo pākahi ʻia nā ʻano ES-PLE a pau e pili ana i nā kūlana hopena kūpono like ʻole.
Laʻana 1 (Biosynthesis o Pregabalin Intermediate)(1):
Laʻana 2(2):
Laʻana 3(3):
Laʻana 4(4):
E mālama no 2 mau makahiki ma lalo o -20℃.
Mai hoʻopā aku me nā kūlana koʻikoʻi e like me: ke kiʻekiʻe o ka mahana, ka pH kiʻekiʻe/haʻahaʻa.
1. Xu FX, Chen SY, Xu G, a me tal. Appl. Biotechnol Bioproc E, 1988, 54(4): 1030.
2. Huang, FC, Lee, LF, Mittal, RSD e pili ana. J. Am. Chem.Soc, 1975, 97, 4144.
3. Kielbasinski, P., Goralczyk, P., Mikolajczyk, M., e tal. Synlett, 1994, 127.
4. Gais, HJ, Griebel, C., Buschmann, H. Tetrahedron: Asymmetry 2000, 11, 917
