Esterase&Lipase (PLE&CALB)
He 26 ʻano huahana enzyme PLE (Helu e like me ES-PLE-101～ES-PLE-126) i hoʻomohala ʻia e SyncoZymes.Hiki ke hoʻohana ʻia ka ES-PLE no ka hydrolysis o nā pūhui aliphatic a me ka ester, a i ʻole regioselective a me stereoselective hoʻonā e synthesize i nā ʻakika chiral a me kā lākou derivatives.
ʻAno hoʻopā catalytic:
★ High substrate specificity.
★ Ke koho kiral ikaika.
★ hoʻololi kiʻekiʻe.
★ Liʻiliʻi nā huahana.
★ Nā kūlana hoʻonā haʻahaʻa.
★ Pili kaiapuni.
➢ ʻO ka mea maʻamau, pono e hoʻokomo ʻia ka ʻōnaehana hoʻololi i ka substrate, ka solution buffer a me ka ES-PLE.Hoʻokō ʻia ka esterification o kekahi mau ES-PLE ma ka pae organik.
➢ Pono e aʻo pākahi ʻia nā ʻano ES-PLE a pau e pili ana i nā ʻano hopena maikaʻi loa.
Laʻana 1(Biosynthesis of Pregabalin Intermediate)(1):
Laʻana 2(2):
Laʻana 3(3):
Laʻana 4(4):
E mālama i 2 mau makahiki ma lalo o -20 ℃.
Mai hoʻopili i nā kūlana koʻikoʻi e like me: ka wela kiʻekiʻe, pH kiʻekiʻe/haʻahaʻa.
1. Xu FX, Chen SY, Xu G, e tal.Appl.Biotechnol Bioproc E, 1988, 54(4): 1030.
2. Huang, FC, Lee, LF, Mittal, RSD e pili ana.J. Am.Chem.Soc, 1975, 97, 4144.
3. Kielbasinski, P., Goralczyk, P., Mikolajczyk, M., e tal.Synlett, 1994, 127.
4. Gais, HJ, Griebel, C., Buschmann, H. Tetrahedron: Asymmetry 2000, 11, 917
