Ketoreductase (KRED)
ʻO KRED mai SyncoZymes: Aia he 191 mau ʻano huahana enzyme KRED (Helu e like me ES-KRED-101 ~ ES-KRED-291) i hoʻomohala ʻia e SyncoZymes. Hiki ke hoʻohana nui ʻia ʻo SZ-KRED i ka hōʻemi regio- a me stereoselective o nā aldehydes, beta-ketone esters, α-ketone esters a me ketones.
ʻAno hana catalytic:
| Nā ʻenzyme | Kōmike Huahana | Nā kikoʻī |
| Pauka ʻenzyme | Pauka ʻenzyme | he hoʻonohonoho o 187 Ketoreductases, 50 mg kēlā me kēia 187 mau mea * 50mg / mea, a i ʻole ka nui ʻē aʻe |
| Pahu Nānā (SynKit) | ES-KRED-18100 | he hoʻonohonoho o 181 Ketoreductases, 1 mg kēlā me kēia 181 mau mea * 1mg / mea |
★ Kiʻekiʻe ke ʻano kikoʻī o ka substrate.
★ Koho chiral ikaika.
★ Ka pono hoʻololi kiʻekiʻe.
★ Emi nā huahana ʻaoʻao.
★ Nā kūlana pane iki.
★ Mālama i ke kaiapuni.
➢ Pono e hoʻokō ʻia ka nānā ʻana o nā enzyme no nā substrates kikoʻī ma muli o ke ʻano kikoʻī o ka substrate, a loaʻa i kahi enzyme e hoʻoulu ai i ka substrate i manaʻo ʻia me ka hopena catalytic maikaʻi loa.
➢ Mai hoʻopā aku me nā kūlana koʻikoʻi e like me: ke kiʻekiʻe o ka mahana, ka pH kiʻekiʻe/haʻahaʻa a me ka mea hoʻoheheʻe organik me ka nui kiʻekiʻe.
➢ ʻO ka maʻamau, pono i ka ʻōnaehana hana ke komo pū me ka substrate, ka hopena buffer, nā coenzymes (NAD(H) a i ʻole NADP(H)), ka ʻōnaehana hana hou ʻana o ka coenzyme (e laʻa me ka glucose a me ka glucose dehydrogenase).
➢ Pono e hoʻohui hope ʻia ʻo KRED i loko o ka ʻōnaehana hana, ma hope o ka hoʻoponopono ʻia ʻana o ka pH a me ka mahana i ke kūlana hana.
➢ Loaʻa i nā ʻano KRED āpau nā kūlana hopena kūpono like ʻole, no laila pono e aʻo hou ʻia kēlā me kēia.
Laʻana 1(1):
Laʻana 2(2):
1. Li Z, Liu WD, Chen X, e tal. Tetrahedron, 2013, 69, 3561-3564.
2. Ni Y, Li CX, Ma HM, e tal. Appl Microbiol Biotechnol, 2011, 89: 1111-1118.
