ʻO ka Lactate Dehydrogenase (LDH)
Wehewehe Pōkole:
E pili ana i ka Lactate dehydrogenase
Aia kekahi ʻano huahana enzyme LDH (Helu e like me ES-LDH) i hoʻomohala ʻia e SyncoZymes. Hiki i ka LDH ke hoʻoikaika i ka hōʻemi ʻana o ka pyruvate i ka waikawa L-lactic, a i ʻole ka hōʻemi ʻana o nā waikawa α-keto like ʻē aʻe. I ke kaʻina hana o ka catalysis, pono ʻo NADH ma ke ʻano he mea lawe hydrogen.
ʻAno hana catalytic lactate dehydrogenase
Kelepona Paʻalima/Wechat/WhatsApp: +86-13681683526
Leka uila:lchen@syncozymes.com
| Nā ʻenzyme | Kōmike Huahana | Nā kikoʻī |
| Pauka ʻenzyme | ES-LDH-101 | he set o 1 Monoamine Oxidase, 50 mg kēlā me kēia 1 mau mea * 50mg / mea, a i ʻole ka nui ʻē aʻe |
Hiki ke hoʻohana ʻia ka ʻōnaehana hana ma lalo nei no ka ʻike:
1-10 mg/ml: Pākuʻi
10 mg/ml: ʻEnzyme
10% (v/v): Mea hoʻoheheʻe organik (1)
100 mM: Palena phosphate (pH7.0)
0.2 mg/ml: NAD+
100 mM: ʻAmonium formate
10 mg/ml: ʻO ka formate dehydrogenase (FDH)
E hoʻomoʻa i nā hopena ma kahi mea lūlū mahana mau (no ka laʻana, 30oC, 150rpm) no 24 ~ 48 mau hola. E centrifuge i kēlā me kēia hui e hoʻoulu i ka protein, hiki ke hoʻohana ʻia ka supernatant no ka nānā ʻana o TLC, HPLC a i ʻole GC.
Nānā (1): He koho ka mea hoʻoheheʻe organik (e like me DMSO, methanol, acetonitrile, IPA, a pēlā aku), hoʻohana ʻia no nā substrates insoluble.
