ʻO ka hoʻemi ʻana o ka nitrile (NRED)
Wehewehe Pōkole:
E pili ana i ka Nitrile reductase
Aia he 3 ʻano huahana enzyme NRED (Helu e like me ES-NRED101 ~ ES-NRED103) i hoʻomohala ʻia e SyncoZymes. Hiki iā NRED ke hoʻoikaika i ka hōʻemi ʻana o ka hui nitrile i amine. I ke kaʻina hana o ka catalysis, pono ʻo NADPH ma ke ʻano he mea lawe hydrogen.
ʻAno hana catalytic Nitrile reductase
Kelepona Paʻalima/Wechat/WhatsApp: +86-13681683526
Leka uila:lchen@syncozymes.com
| Nā ʻenzyme | Kōmike Huahana | Nā kikoʻī |
| Nā kikoʻī | ES- NRED -101~ ES- NRED -103 | he set o 3 Nitrile Reductase, 50 mg kēlā me kēia 3 mau mea * 50mg / mea, a i ʻole ka nui ʻē aʻe |
Hiki ke hoʻohana ʻia ka ʻōnaehana hana ma lalo nei no ka ʻike:
1-10 mg/ml Pākuʻi
10 mg/ml ʻEnzyme
10% (v/v) Mea hoʻoheheʻe organik (1)
100 mM Phosphate buffer (pH7.0)
0.2 mg/ml NADP+
10 mg/ml Glucose
2 mg/ml Glucose dehydrogenase (GDH)
E hoʻomoʻa i nā hopena ma kahi mea lūlū mahana mau (no ka laʻana, 30oC, 150rpm) no 24 ~ 48 mau hola. E centrifuge i kēlā me kēia hui e hoʻoulu i ka protein, hiki ke hoʻohana ʻia ka supernatant no ka nānā ʻana o TLC, HPLC a i ʻole GC.
Nānā (1): He koho ka mea hoʻoheheʻe organik (e like me DMSO, methanol, acetonitrile, IPA, a pēlā aku), hoʻohana ʻia no nā substrates insoluble.
