Phenylalanine ammonia lyase (PAL)
Nā Enzymes | Code Huahana | Hōʻike |
ʻO ka pauka Enzyme | ES-PAL-101~ ES-PAL-110 | he set o 10 L-phenylalanine ammonia-lyase, 50 mg i kēlā me kēia 10 mau mea * 50mg / mea, a i ʻole ka nui ʻē aʻe |
★ High substrate specificity.
★ Ke koho kiral ikaika.
★ hoʻololi kiʻekiʻe.
★ Liʻiliʻi nā huahana.
★ Nā kūlana hoʻonā haʻahaʻa.
★ Pili kaiapuni.
➢ ʻO ka mea maʻamau, pono e hoʻokomo ʻia ka ʻōnaehana hoʻololi i ka substrate, ka solution buffer a me ka enzyme.
➢ ʻO kēlā me kēia ʻano ES-PAL e pili ana i nā ʻano ʻano hopena maikaʻi loa, hiki ke aʻo ʻia pākahi.
➢ Pono e hoʻohui hope ʻia ka ES-PAL i loko o ka ʻōnaehana pane no ka mālama ʻana i ka hana.
Laʻana 1(Biosynthesis o trans cinnamic acid mai phenylalanine)(1):
Laʻana 2(Biosynthesis o ka phenylalanine derivative mai ka trans-cinnamic acid derivative)(2):
E mālama i 2 mau makahiki ma lalo o -20 ℃.
Mai hoʻopili i nā kūlana koʻikoʻi e like me: ka wela kiʻekiʻe, ka pH kiʻekiʻe/haʻahaʻa a me ka mea hoʻoheheʻe kūlohelohe kiʻekiʻe.
1. Zachary Mays, Karishma Mohan,et al.Kemu Commun (Camb).2020, 56, 5255-5258.
2. Ian Rowles, Bas Groenendaal,et al. Tetrahedron,2016. 72, 7343-7347.