Phenylalanine dehydrogenase (PDH)
Nā Enzymes | Code Huahana | Hōʻike |
ʻO ka pauka Enzyme | ES-PDH-101~ ES-PDH-108 | he set o 8 Nitrile Reductase, 50 mg kēlā me kēia 8 mau mea * 50mg / mea, a i ʻole ka nui ʻē aʻe |
★ High substrate specificity.
★ Ke koho kiral ikaika.
★ hoʻololi kiʻekiʻe.
★ Liʻiliʻi nā huahana.
★ Nā kūlana hoʻonā haʻahaʻa.
★ Pili kaiapuni.
➢ ʻO ka mea maʻamau, pono e hoʻokomo i ka ʻōnaehana hoʻololi i kahi substrate, solution buffer, enzyme, coenzyme (NAD(H)), coenzyme regeneration system (eg ammonium formate a me formate dehydrogenase).
➢ ʻO kēlā me kēia ʻano ES-PDH e pili ana i nā ʻano ʻano hopena maikaʻi loa, hiki ke aʻo ʻia kēlā me kēia inā pono.
➢ Pono e hoʻohui hope ʻia ka ES-PDH i loko o ka ʻōnaehana pane no ka mālama ʻana i ka hana.
Ka Laʻana 1(Biosynthesis o nā mea hoʻoheheʻe phenylalanine mai nā mea hoʻoheheʻe phenylpyruvate)(1):
E mālama i 2 mau makahiki ma lalo o -20 ℃.
Mai hoʻopili i nā kūlana koʻikoʻi e like me: ka wela kiʻekiʻe, ka pH kiʻekiʻe/haʻahaʻa a me ka mea hoʻoheheʻe kūlohelohe kiʻekiʻe.
1. ʻO Francesca Paradisi, Stuart Collins, et al.Nūpepa o Biotechnology, 2007, 128, 408–411.