Kiʻi i hōʻike ʻia ʻo Cytochrome P450 monooxygenase (CYP)

ʻO ka Cytochrome P450 monooxygenase (CYP)

Wehewehe Pōkole:

E pili ana iā CYP

ES-CYPs: He papa o nā enzymes redox me mercaptan-heme ma ke kikowaena hana e hoʻoulu ai i ka hoʻoili oxygen. He mau oxygenases hope lākou a pono i ka heme e hoʻoulu i ka oxygen.

Hoʻoulu ka Cytochrome P450 monooxygenase i ka oxidation, epoxidation a me ka hydroxylation o ka alkyl, hydroxylation a me ka oxidation o ka ammonia, oxidation o ka sulfur, dealkylation (oxygen, sulfur, ammonia), oxidative dehydrogenation, deamination a me ka dehalogenation.
Aia he 8 ʻano o nā huahana cytochrome P450 monooxygenase (Helu e like me ES-CYP-101～ES-CYP-108) i hoʻomohala ʻia e SyncoZymes.

Kelepona Paʻalima/Wechat/WhatsApp: +86-13681683526

Leka uila:lchen@syncozymes.com

E leka uila mai iā mākou

Nā kikoʻī huahana

Nā Lepili Huahana

E pili ana iā CYP:

ʻAno hana catalytic:

ʻIke Huahana:

Nā ʻenzyme	Pahu Nānā (SynKit)	Nā kikoʻī
Pauka ʻenzyme	ES-CYP-101~ ES-CYP-108	he seti o 8 Cytochrome P450 Monooxygenases, 50 mg kēlā me kēia 8 mau mea * 50mg / mea, a i ʻole ka nui ʻē aʻe
Pahu Nānā (SynKit)	ES-CYP-800	he set o 8 Cytochrome P450 Monooxygenases,1mg kēlā me kēia 8 mau mea * 1mg / mea

Nā Pōmaikaʻi:

★ Ka laulā o ka substrate.
★ Hoʻololi kiʻekiʻe.
★ Emi nā huahana ʻaoʻao.
★ Nā kūlana pane iki.
★ Mālama i ke kaiapuni.

Nā kuhikuhi no ka hoʻohana ʻana:

➢ ʻO ka maʻamau, pono i ka ʻōnaehana hana ke komo pū me ka substrate, ka hopena buffer (ʻO ka pH hana kūpono o ka enzyme), coenzyme (NAD(H) a i ʻole NADP(H)), ka ʻōnaehana hana hou ʻana o ka coenzyme (e laʻa me ka glucose a me ka glucose dehydrogenase) a me ka ES-CYP. hiki ke pani ʻia ka ʻōnaehana hana hou ʻana o ka coenzyme a me ka coenzyme e ka hydrogen peroxide i loko o ka ʻōnaehana hana hapa.
➢ Pono e aʻo pākahi ʻia nā ʻano ES-CYP āpau e pili ana i nā kūlana hopena kūpono like ʻole.
➢ Hiki i ka substrate a i ʻole ka huahana kiʻekiʻe ke kāohi i ka hana a ES-CYP. Eia nō naʻe, hiki ke hoʻomaha ʻia ke kāohi ʻana ma o ka hoʻohui ʻana o ka substrate.

Nā Laʻana Noi:

Laʻana 1⁽¹⁾:

Laʻana 2⁽²⁾:

Laʻana 3⁽³⁾:

Laʻana 4⁽⁴⁾:

Ka mālama ʻana:

E mālama no 2 mau makahiki ma lalo o -20℃.

Hoʻolohe:

Mai hoʻopili aku me nā kūlana koʻikoʻi e like me: ke kiʻekiʻe o ka mahana, ka pH kiʻekiʻe/haʻahaʻa a me ka mea hoʻoheheʻe organik kiʻekiʻe.

Nā Kuhikuhi:

1. Zaretzki J, Matlock M, a me Swamidass S JJ Chem. Inf. Model, 2013, 53, 3373–3383.
2. Gannett P M., Kabulski J, Perez F A., e tal. J. Am. Kemi. Soc., 2006, 128 (26), 8374–8375.
3. ʻO Cryle M J., ʻo Matovic N J., a me De Voss J J. Org. Lett., 2003, 5 (18), 3341–3344.
4. Kawauchi, H., Sasaki, J., Adachi, T., e tal. Biochim. Biophys. Acta, 1994, 1219, 179.
5. Yasutake, Y., Fujii, Y.; Cheon, WK e tal. Acta Crystallogr. 2009, 65, 372.