Epoxide hydrolase (EH)
ʻO ka wehewehe pōkole:
E pili ana i ka epoxide hydrolase
ʻO ka EH ka eter hydrolase e hoʻopololei i ka stereoselective hydrolysis o racemic epoxides e hana i ka 1, 2-diol a me ka optically active epoxides, hiki ke hoʻohana ʻia no ka hoʻonā chiral o nā epoxides a i ʻole ka hoʻomākaukau ʻana i nā diols chiral.Hoʻohana nui ʻia lākou i ka lāʻau lapaʻau a me nā ʻoihana ʻē aʻe.Aia 3 ʻano huahana epoxide hydrolases (Helu e like me ES-EH-101～ES-EH-103) i hoʻomohala ʻia e SyncoZymes.He mea hana pono ka ES-EH no ka hoʻopili ʻana i ka synthesis o nā waiʻona chiral ma o ka stereoselective hydrolysis o aliphatic a me nā epoxide aromatic.
ʻAno hoʻopā catalytic:
Mobile/Wechat/WhatsApp: +86-13681683526
E-mail:lchen@syncozymes.com
| Nā Enzymes | Code Huahana | Hōʻike |
| ʻO ka pauka Enzyme | ES-EH-101~ ES-EH-103 | kahi hoʻonohonoho o 3 Epoxide Hydrolases, 50 mg kēlā me kēia 3 mau mea * 50mg / mea, a i ʻole ka nui ʻē aʻe |
| Mea Hoʻomaʻamaʻa (SynKit) | ES-EH-300 | kahi hoʻonohonoho o 3 Epoxide Hydrolases, 1 mg i kēlā me kēia 3 mau mea * 1mg / mea |
★ High substrate specificity.
★ Ke koho kiral ikaika.
★ hoʻololi kiʻekiʻe.
★ Liʻiliʻi nā huahana.
★ Nā kūlana hoʻonā haʻahaʻa.
★ Pili kaiapuni.
➢ ʻO ka mea maʻamau, pono e hoʻokomo i ka ʻōnaehana hoʻololi i ka substrate, ka solution buffer (pH hopena maikaʻi loa) a me ES-EH.
➢ Pono e aʻo pākahi ʻia nā ʻano ES-EH a pau e pili ana i nā ʻano ʻano hopena maikaʻi loa.
➢ Hiki ke kaohi i ka hana a ES-EH.Eia naʻe, hiki ke hoʻokuʻu ʻia ka inhibition me ka hoʻohui pū ʻana o ka substrate.
Laʻana 1(1):
E mālama i 2 mau makahiki ma lalo o -20 ℃.
Mai hoʻopili i nā kūlana koʻikoʻi e like me: ka wela kiʻekiʻe, ka pH kiʻekiʻe/haʻahaʻa a me ka mea hoʻoheheʻe kūlohelohe kiʻekiʻe.
1. Kim YH, Lee I, Choi SH, e tal.J Mol Catal B-Enzym, 2013, 89: 48-51.
