ʻEpoxide hydrolase (EH)
Wehewehe Pōkole:
E pili ana i ka epoxide hydrolase
ʻO EH ka ether hydrolase e hoʻoulu ai i ka hydrolysis stereoselective o nā racemic epoxides e hana i ka 1, 2-diol a me nā epoxides optically active, hiki ke hoʻohana ʻia no ka hoʻonā chiral o nā epoxides a i ʻole ka hoʻomākaukau ʻana o nā chiral diols. Hoʻohana nui ʻia lākou i ka lāʻau lapaʻau a me nā ʻoihana ʻē aʻe. Aia he 3 ʻano o nā huahana epoxide hydrolases (Helu e like me ES-EH-101～ES-EH-103) i hoʻomohala ʻia e SyncoZymes. He mea hana pono ʻo ES-EH e hoʻoulu ai i ka synthesis o nā chiral alcohols ma o ka hydrolysis stereoselective o nā aliphatic a me nā aromatic epoxides.
ʻAno hana catalytic:
Kelepona Paʻalima/Wechat/WhatsApp: +86-13681683526
Leka uila:lchen@syncozymes.com
| Nā ʻenzyme | Kōmike Huahana | Nā kikoʻī |
| Pauka ʻenzyme | ES-EH-101~ ES-EH-103 | he set o 3 Epoxide Hydrolases, 50 mg kēlā me kēia 3 mau mea * 50mg / mea, a i ʻole ka nui ʻē aʻe |
| Pahu Nānā (SynKit) | ES-EH-300 | he set o 3 Epoxide Hydrolases, 1 mg kēlā me kēia 3 mau mea * 1mg / mea |
★ Kiʻekiʻe ke ʻano kikoʻī o ka substrate.
★ Koho chiral ikaika.
★ Hoʻololi kiʻekiʻe.
★ Emi nā huahana ʻaoʻao.
★ Nā kūlana pane iki.
★ Mālama i ke kaiapuni.
➢ ʻO ka maʻamau, pono i ka ʻōnaehana hana ke komo pū me ka substrate, ka hopena buffer (pH hana kūpono loa) a me ES-EH.
➢ Pono e aʻo pākahi ʻia nā ʻano ES-EH āpau e pili ana i nā kūlana hopena kūpono like ʻole.
➢ Hiki i ka substrate a i ʻole ka huahana kiʻekiʻe ke kāohi i ka hana a ES-EH. Eia nō naʻe, hiki ke hoʻomaha ʻia ke kāohi ʻana ma o ka hoʻohui ʻana o ka substrate.
Laʻana 1(1):
E mālama no 2 mau makahiki ma lalo o -20℃.
Mai hoʻopili aku me nā kūlana koʻikoʻi e like me: ke kiʻekiʻe o ka mahana, ka pH kiʻekiʻe/haʻahaʻa a me ka mea hoʻoheheʻe organik kiʻekiʻe.
1. Kim YH, Lee I, Choi SH, e tal. J Mol Catal B-Enzym, 2013, 89: 48-51.
