ʻO ka nitrile hydratase (NHT)
Wehewehe Pōkole:
E pili ana i nā ES-NHT
ʻO ES-NHTs: kahi papa o ka metalloenzyme, kahi e hoʻokahe koho ai i nā nitriles i loko o ka amide mua e pili ana. Hoʻohana nui ʻia lākou ʻaʻole wale no ka synthesis o nā waikawa amino, amides, nā waikawa carboxylic a me kā lākou derivatives, akā no ka hoʻomākaukau ʻana i nā lāʻau chiral. Aia he 24 mau ʻano nitrile hydratases i hoʻomohala ʻia e SyncoZymes (Helu e like me ES-NHT-101～ES-NHT-124).
ʻAno hana catalytic:
Kelepona Paʻalima/Wechat/WhatsApp: +86-13681683526
Leka uila:lchen@syncozymes.com
| Nā ʻenzyme | Kōmike Huahana | Nā kikoʻī |
| Pauka ʻenzyme | ES-NHT-101~ ES-NHT-124 | he set o 24 Nitrile Hydratases, 50 mg kēlā me kēia 24 mau mea * 50mg / mea, a i ʻole ka nui ʻē aʻe |
| Pahu Nānā (SynKit) | ES-NHT-2400 | he set o 24 Nnitrile Hydratases, 1 mg kēlā me kēia 24 mau mea * 1mg / mea |
★ Kiʻekiʻe ke ʻano kikoʻī o ka substrate.
★ Koho chiral ikaika.
★ Hoʻololi kiʻekiʻe.
★ Emi nā huahana ʻaoʻao.
★ Nā kūlana pane iki.
★ Mālama i ke kaiapuni.
➢ ʻO ka maʻamau, pono i ka ʻōnaehana hana ke komo pū me ka substrate, ka hopena buffer (pH hana kūpono loa) a me ES-NHT.
➢ Hiki ke hoʻāʻo ʻia nā ES-NHT āpau ma ka ʻōnaehana hopena ma luna a i ʻole me ka NHT Screening Kit (SynKit NHT).
➢ Pono e aʻo pākahi ʻia nā ʻano ES-NHT āpau e pili ana i nā kūlana hopena kūpono like ʻole.
➢ Hiki i ka substrate a i ʻole ka huahana kiʻekiʻe ke kāohi i ka hana a ES-NHT. Eia nō naʻe, hiki ke hoʻomaha ʻia ke kāohi ʻana ma o ka hoʻohui ʻana o ka substrate, me ka hui pū ʻana me nā amidase a i ʻole ka unuhi huahana.
Laʻana 1 (hoʻopili ʻana me ka amidase)(1):
Laʻana 2(2):
E mālama no 2 mau makahiki ma lalo o -20℃.
Mai hoʻopili aku me nā kūlana koʻikoʻi e like me: ke kiʻekiʻe o ka mahana, ka pH kiʻekiʻe/haʻahaʻa a me ka mea hoʻoheheʻe organik kiʻekiʻe.
1 Vojtech V, Ludmila M, Alicja BV, a me tal. J Mol Catal B-enzym, 2011, 71: 51-55.
2 Gary W. B, Thomas G, Christopher B. M, a me tal. Tetrahedron lett, 2010, 51: 1639-1641.
