Nitrilase (NIT)
Wehewehe Pōkole:
E pili ana iā Nitrilase
ES-NITs: Hoʻololi pololei i ka hui cyanide i ka hui carboxyl, e hoʻomākaukau i ka waikawa carboxylic, i hoʻohana nui ʻia i ka lāʻau lapaʻau, ka palekana o ke kaiapuni, ka ʻoihana kemika, ka mea hoʻohui meaʻai, ka lole, ka hana mahiʻai a me nā ʻoihana ʻē aʻe.
Aia he 40 ʻano o nā huahana Nitrilase (NIT) (Helu e like me ES-NIT-101 ~ ES-NIT-140) i hoʻomohala ʻia e SyncoZymes. He mea hana pono ʻo ES-NIT e hoʻoulu ai i ka synthesis o nā waikawa carboxylic chiral ma o ka regio- a me ka stereoselective hydrolysis o kahi ākea o nā nitriles aliphatic a me nā aromatic.
ʻAno hana catalytic:
Kelepona Paʻalima/Wechat/WhatsApp: +86-13681683526
Leka uila:lchen@syncozymes.com
| Nā ʻenzyme | Kōmike Huahana | Nā kikoʻī |
| Pauka ʻenzyme | ES-NIT-101~ ES-NIT-140 | he set o 40 Nitrilase, 50 mg kēlā me kēia 40 mau mea * 50mg / mea, a i ʻole ka nui ʻē aʻe |
| Pahu Nānā (SynKit) | ES-NIT-4000 | he set o 40 Nitrilase, 1 mg kēlā me kēia 40 mau mea * 1mg / mea |
★ Kiʻekiʻe ke ʻano kikoʻī o ka substrate.
★ Hoʻololi kiʻekiʻe.
★ Emi nā huahana ʻaoʻao.
★ Nā kūlana pane iki.
★ Mālama i ke kaiapuni.
➢ ʻO ka maʻamau, pono i ka ʻōnaehana hana ke komo pū me ka substrate, ka hopena buffer (pH hana kūpono loa) a me ES-NIT.
➢ Hiki ke hoʻāʻo ʻia nā ES-NIT āpau ma ka ʻōnaehana hopena ma luna a i ʻole me ka NIT Screening Kit (SynKit NIT).
➢ Pono e aʻo pākahi ʻia nā ʻano ES-NIT āpau e pili ana i nā kūlana hopena kūpono like ʻole.
➢ Hiki i ka substrate a i ʻole ka huahana kiʻekiʻe ke kāohi i ka hana a ES-NIT. Eia nō naʻe, hiki ke hoʻomaha ʻia ke kāohi ʻana ma o ka hoʻohui ʻana o ka substrate.
Laʻana 1 (Hana ʻia ʻana o ka waikawa nicotinic)(1):
Laʻana 2 (Hana ʻia o ka waikawa (R)-(-)-mandelic)(2):
Laʻana 3 (Hana ʻia ʻana o ka baclofen)(3):
Laʻana 4 (Hana ʻia ka waikawa glycolic)(4):
E mālama no 2 mau makahiki ma lalo o -20℃.
Mai hoʻopili aku me nā kūlana koʻikoʻi e like me: ke kiʻekiʻe o ka mahana, ka pH kiʻekiʻe/haʻahaʻa a me ka mea hoʻoheheʻe organik kiʻekiʻe.
1 Mathew CD, Nagasawa T, Kobayashi M, a me tal. Appl. Kaiapuni. Microbiol, 1988, 54(4): 1030.
2 Yamamoto K, Oishi K, Fujimatsu I, e tal. Appl. Kaiapuni. Microbiol, 1991, 57(10): 3028.
3 Xu MZ, Ren J, Gong JS, a me ka tal. Chin. J. Biotechnol, 2013, 29(1): 31.
4 Wu S, Fogiel AJ, Petrillo KL, e tal. Biotechnol. Bioeng., 2008, 99(3): 717.
