ʻO Phosphokinase (PKase)
Wehewehe Pōkole:
E pili ana i ka Phosphokinase
ES-PKase (Phosphokinase): hoʻoulu i ka hoʻoili ʻana o nā hui phosphate ma ATP i nā hui ʻē aʻe. Hoʻoulu pū ia i ka hoʻoili ʻana o nā hui phosphate ma nā nucleosides ʻē aʻe o ka triphosphate i kekahi manawa. Pono ka hapa nui o nā kinases i nā ion metala divalent e komo i ka hopena (ma ke ʻano maʻamau ʻo Mg2+). Aia he 21 ʻano o nā huahana enzyme PKase (Helu e like me ES-PKase101 ~ ES-PKase-121) i hoʻomohala ʻia e SyncoZymes.
ʻAno hana catalytic:
Kelepona Paʻalima/Wechat/WhatsApp: +86-13681683526
Leka uila:lchen@syncozymes.com
| Nā ʻenzyme | Kōmike Huahana | Nā kikoʻī |
| Pahu Nānā (SynKit) | ES-PKase-101~ES-PKase-121 | he hoʻonohonoho o 21 Ketoreductases, 1 mg kēlā me kēia 21 mau mea * 1mg / mea |
★ Kiʻekiʻe ke ʻano kikoʻī o ka substrate.
★ Koho chiral ikaika.
★ Hoʻololi kiʻekiʻe.
★ Emi nā huahana ʻaoʻao.
★ Nā kūlana pane iki.
★ Mālama i ke kaiapuni.
➢ Ma ke ʻano maʻamau, pono e hoʻokomo pū ka ʻōnaehana hana i ka substrate, ka hopena buffer, ka enzyme, ATP, Mg2+.
➢ Pono e hoʻohui hope ʻia ʻo PKase i loko o ka ʻōnaehana hana, ma hope o ka hoʻoponopono ʻia ʻana o ka pH a me ka mahana i ke kūlana hana.
Laʻana 1 (Hana ʻia o ka nicotinamide ribose phosphate mai ka nicotinamide ribose)(1):
Nānā: ua manaʻo ʻia nā laʻana noi a me nā kuhikuhi e hōʻike i ka laulā o ka noi o PKase no ka maʻalahi o ka hoʻomaopopo ʻana, a ʻaʻole kūlike me ka enzyme kikoʻī o SyncoZymes.
E mālama no 2 mau makahiki ma lalo o -20℃.
Mai hoʻopili aku me nā kūlana koʻikoʻi e like me: ke kiʻekiʻe o ka mahana, ka pH kiʻekiʻe/haʻahaʻa a me ka mea hoʻoheheʻe organik kiʻekiʻe.
1. Khan, Javed A., Song Xiang, a me Liang Tong. ʻAno 15.8 (2007): 1005-1013.
