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Imine reductase (IRED)
E pili ana iā Imine reductase
ES-IRED: He papa o nā enzymes nāna e hoʻoemi i ka hoʻopaʻa C=N i ka paʻa CN.No ka redox enzyme, me ka coenzyme NADPH no ka lawe hydrogen.Wahi a ka makemake chiral o nā huahana, hiki ke hoʻokaʻawale ʻia i R-IRED a me S-IRED.Hoʻokumu ʻo Syncozymes i 15 imine reductases (numbIRED ES-IRED-101-ES-IRED-115), hiki ke hoʻohana nui ʻia i ka regioselective a me ka stereoselective reduction o nā imines e hoʻomākaukau ai i nā amine chiral.
ʻAno hoʻopā catalytic:
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Nitrile reductase (NRED)
E pili ana i ka Nitrile reductase
Aia 3 ʻano huahana enzyme NRED (Helu e like me ES-NRED101~ ES-NRED103) i hoʻomohala ʻia e SyncoZymes.Hiki i ka NRED ke ho'ēmi i ka hui nitrile i amine.I ke kaʻina hana catalysis, pono ʻo NADPH ma ke ʻano he hydrogen transporter.
Nitrile reductasecatalytic reaction type
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Phenylalanine dehydrogenase (PDH)
E pili ana i ka Phenylalanine dehydrogenase
ES-PDH: He papa o oxidoreductase.Hiki i ka hopena maikaʻi ke hoʻopau i ka deamination oxidative o ka paʻakai L phenylalanine i mua o ka NAD e hana i ka paʻakai phenylpyruvate, a hiki i ka hopena hope ke hoʻoheheʻe i ka synthesis o ka paʻakai amino acid mai ka paʻakai phenylpyruvate.Hoʻokumu ʻo Syncozymes i 8 mau mea o ka phenylalanine dehydrogenase (heluhelu ES-PDH-101~ES-PDH-108).Hiki ke hoʻohana ʻia ka ES-PDH no ka hoʻomākaukau ʻana i ka paʻakai phenylalanine a i ʻole ka paʻakai ʻakika amino e pili ana mai ka paʻakai ʻakika phenylpyruvic a i ʻole nā mea waiwai.
ʻAno hoʻopā catalytic:
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Leucine Dehydrogenase (LeuDH)
E pili ana i ka Leucine dehydrogenase
Aia 1 ʻano huahana enzyme LeuDH (Helu e like me ES-LeuDH-101) i hoʻomohala ʻia e SyncoZymes.Hiki i ka LeuDH ke hoʻololi i ka ammoniation reductive o ka 4-methylbutanoic acid (paʻakai) i ka L-leucine a i ʻole ka oxidation o ka leucine i ka 4-methylbutanoic acid.Hiki i ka ES-LeuDH ke hoʻoikaika i ka hoʻohaʻahaʻa ʻana o nā ʻakika α-keto me ke ʻano like e hoʻomākaukau ai i nā derivatives amino acid.I ke kaʻina hana o ka catalysis, pono ʻo NADH ma ke ʻano he hydrogen transporter.
Leucine dehydrogenasecatalytic reaction type
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Threonine deaminase (TDA)
E pili ana i ka Threonine deaminase
ʻO ES-TDA (Threonine deaminase): kahi protein pyridoxal phosphate e hoʻokaʻawale i ka deamination o threonine e hana i ka 2-ketobutyric acid a me ka amonia.Pono ʻo Pyridoxal 5-phosphate ma ke ʻano he coenzyme i ka ʻōnaehana catalytic.Hoʻokahi wale nō ʻano huahana enzyme TDA (Number as ES-TDA) i hoʻomohala ʻia e SyncoZymes, hiki ke hoʻohana ʻia no ka hoʻomākaukau ʻana i ka waikawa 2-ketobutyric a i ʻole nā mea i loaʻa mai ka threonine.
ʻAno hoʻopā catalytic:
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Amine Dehydrogenase (AmDH)
E pili ana iā Amine Dehydrogenase
ES-AmDH (Amine Dehydrogenase): he enzyme e hiki ke hoʻokahe i ka ammoniation o ka hui kalapona a hoʻemi ia i ka hui amino.He enzyme hilinaʻi coenzyme NADH, a hana ʻo NADH ma ke ʻano he hydrogen transmitter.He 4 mau ʻano huahana enzyme AmDH (Helu e like me ES-AmDH101～ES-AmDH104) i hoʻomohala ʻia e SyncoZymes, hiki ke hoʻohana ʻia i ka regioselective a i ʻole stereoselective reductive amination o nā aldehydes a me nā ketones.
ʻAno hoʻopā catalytic:
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Nucloside phosphoryalse (NP)
E pili ana i ka nucleoside phosphoryalse
Aia 3 ʻano huahana enzyme NP (Helu e like me ES-NP-101~ ES-NP-103) i hoʻomohala ʻia e SyncoZymes.ʻO ES-NP-101 ka purine nucleoside phosphorylase, ES-NP-102 a me ES-NP-103 he pyrimidine nucleoside phosphorylase.Hiki i ka Nucleosidephosphorylase ke hoʻoheheʻe i nā nucleosides i nā kumu a me ka pentose phosphate.Hiki ke hoʻokaʻawale ʻia ka Nucleoside phosphorylase i purine nucleoside phosphorylase a me pyrimidine nucleoside phosphorylase e like me ka makemake o nā kumu nucleoside.Hiki i ka purine nucleoside phosphorylase ke hoʻololi i ka adenosine i adenine, inosine i hypoxanthine, guanosine i guanine, a hana i ka ribose phosphate i ka manawa like.Hiki i ka Pyrimidine nucleoside phosphorylase ke hoʻololi i ka uridine i ka uracil a hana i ka ribose phosphate.
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Hydroaminase (HAM)
E pili ana i ka Hydroaminase
Aia 2 ʻano huahana enzyme HAM (Helu e like me ES-HAM-101~ ES-HAM-102) i hoʻomohala ʻia e SyncoZymes.Hiki i ka HAM ke hoʻokaʻawale i ka ammoniation o ka waika enoic a i ʻole nā mea i loaʻa mai e hana i nā waikawa amino chiral.Hiki ke hoʻohana ʻia ka HAM e hoʻohui i nā ʻakika amino chiral (a i ʻole chiral amines) mai nā waikawa enoic (a i ʻole alkenes).Pono ka mea hāʻawi Ammonia, e like me ka wai amonia a i ʻole ka paʻakai ammonium.
Hydroaminase catalytic pane ʻano
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Hoʻokuʻu ʻia ʻo CALB
CALB
Hana ʻia ʻo Recombinant Lipase B mai Candida antarctica (CALB) e ka fermentation submerged me Pichia pastoris i hoʻololi ʻia.
Hiki ke hoʻohana ʻia ʻo CALB i loko o ka wai a i ʻole ka organik phase catalytic esterification, esterolysis, transesterification, ring opening polyester synthesis, aminolysis, hydrolysis of amides, acylation of amines a me ka hoʻohui ʻana.
Loaʻa ka CALB me ke koho chiral kiʻekiʻe a me ke koho kūlana, no laila hiki ke hoʻohana nui ʻia i ka hana ʻaila, ka meaʻai, ka lāʻau lapaʻau, nā mea hoʻonaninani a me nā ʻoihana kemika ʻē aʻe.
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NADH oxidase (NOX)
E pili ana i ka NADH oxidase
ES-NOX (NADH oxidase): NOX hoʻopale i ka oxidation o NADH i NAD +, a no ka oxidoreductase.I ke kaʻina o ka oxidation, pono ʻo O2 ma ke ʻano he oxidant, a hoʻemi ʻia i H2O a i ʻole H2O2.Aia he 4 mau ʻano huahana enzyme NOX (ES-NOX-101~ES-NOX-104) i hoʻomohala ʻia e kā mākou hui, hiki ke hoʻohana ʻia no ka hoʻōla hou ʻana o ka coenzyme NAD +.
ʻAno hoʻopā catalytic:
or
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Phenylalanine ammonia lyase (PAL)
No Phenylalanine ammonia lyase
ES-PAL: He papa o nā enzymes e hoʻopololei i ka hoʻokaʻawale pololei ʻana o L-phenylalanine i ka waikawa transcinnamic.Hoʻokumu ʻo Syncozymes i 10 mau mea o ka phenylalanine ammonia lyase (helu ʻia ʻo ES-PAL-101~ES-PAL-110), hiki ke hoʻohana ʻia i ka deamination a i ʻole ka hoʻohuli ʻana o ka phenylalanine a me kāna mau mea.
ʻAno hoʻopā catalytic:
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Catalase (CAT)
No Catalase
ES-CAT (Catalase): hoʻopaʻa i ka decomposition o H2O2 i oxygen a me ka wai.Loaʻa ia ma ka mitochondria, endoplasmic reticulum, nā holoholona a me nā ʻulaʻula ʻulaʻula, ʻoi aku hoʻi i loko o ke ake i ke kiʻekiʻe kiʻekiʻe, e hāʻawi ana i ka hana pale antioxidant no ke kino.I ka biocatalysis, hoʻohana nui ia e wehe i ka hopena o ka hydrogen peroxide a hoʻemi i ka inhibition a me ka inactivation o ka enzyme e ka hydrogen peroxide.Hoʻokahi wale nō ʻano huahana enzyme CAT i hoʻomohala ʻia e SyncoZymes (Number like ES-CAT).
ʻAno hoʻopā catalytic:
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E-Mail:lchen@syncozymes.com
